SMRT sequencing of the full-length transcriptome of the Rhynchophorus ferrugineus (Coleoptera: Curculionida)
文献类型： 外文期刊
作者： Yang, Hongjun 1 ; Xu, Danping 2 ; Zhuo, Zhihang 1 ; Hu, Jiameng 1 ; Lu, Baoqian 4 ;
作者机构： 1.Hainan Univ, Coll Forestry, Key Lab Genet & Germplasm Innovat Trop Special Fo, Minist Educ,Key Lab Germplasm Resources Biol Trop, Haikou, Hainan, Peoples R China
2.Sichuan Agr Univ, Coll Food Sci, Sichuan Prov Key Lab Agr Prod Proc & Preservat, Yaan, Sichuan, Peoples R China
3.South China Agr Univ, Key Lab Integrated Pest Management Crops South Ch, Minist Agr, Guangzhou, Guangdong, Peoples R China
4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr China, Haikou, Hainan, Peoples R China
关键词： Rhynchophorus ferrugineus; Different developmental stage; PacBio Iso-Seq; Illumina RNA-seq; Full-length transcriptome
期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）
ISSN： 2167-8359
年卷期： 2020 年 8 卷
页码：
收录情况： SCI
摘要： Background. Red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is one of the most destructive insects for palm trees in the world. However, its genome resources are still in the blank stage, which limits the study of molecular and growth development analysis. Methods. In this study, we used PacBio Iso-Seq and Illumina RNA-seq to first generate transcriptome from three developmental stages of R. ferrugineus (pupa, 7th larva, female and male) to increase our understanding of the life cycle and molecular characteristics of R. ferrugineus. Results. A total of 63,801 nonredundant full-length transcripts were generated with an average length of 2,964 bp from three developmental stages, including the 7th instar larva, pupa, female adult and male adult. These transcripts showed a high annotation rate in seven public databases, with 54,999 (86.20%) successfully annotated. Meanwhile, 2,184 alternative splicing (AS) events, 2,084 transcription factors (TFs), 66,230 simple sequence repeats (SSR) and 9,618 Long noncoding RNAs (IncRNAs) were identified. In summary, our results provide a new source of full-length transcriptional data and information for the further study of gene expression and genetics in R. ferrugineus.
- 相关文献
作者其他论文 更多>>
-
Control of postharvest stem-end rot on mango by antifungal metabolites of Trichoderma pinnatum LS029-3
作者：Zhan, Xin;Khan, Raja Asad Ali;Zhang, Jingya;Yin, Yaping;Wang, Rui;Liu, Tong;Chen, Jie;Yin, Yaping;Tang, Zhengbin;Lu, Baoqian;Lu, Baoqian;Liu, Tong
关键词：Lasiodiplodia theobromae; Botryodiplodia theobromae; fruit; non-volatile metabolites; GCMS; SEM
-
Characterization and analysis of the transcriptome in Opisina arenosella from different developmental stages using single-molecule real-time transcript sequencing and RNA-seq
作者：Xu, Danping;Zhuo, Zhihang;Yang, Hongjun;Zhuo, Zhihang;Hu, Jiameng;Yang, Fan;Lu, Baoqian
关键词：Single-molecule real-time sequencing; Illumina RNA sequencing; Opisina arenosella
-
Influence of climatic factors on the potential distribution of pest Heortia vitessoides Moore in China
作者：Xu, Danping;Li, Xinyang;Jin, Yawen;Zhuo, Zhihang;Yang, Hongjun;Hu, Jiameng;Xu, Danping;Xu, Danping;Zhuo, Zhihang;Wang, Rulin
关键词：Heortia vitessoides Moore; Potential distribution area; Climatic factors; MaxEnt modeling
-
Inter-country trade, genetic diversity and bio-ecological parameters upgrade pest risk maps for the coconut hispid Brontispa longissima
作者：Lu, Baoqian;Peng, Zhengqiang;Lu, Hui;Lin, Peiqun;Chu, Xiaoqiang;He, Xing;Tang, Jihong;Yang, Fan
关键词：invasive beetles; Brontispa longissima; global trade; biological trait; invasion biology
-
Transcriptome and gene expression analysis of Rhynchophorus ferrugineus (Coleoptera: Curculionidae) during developmental stages
作者：Yang, Hongjun;Xu, Danping;Zhuo, Zhihang;Yang, Hongjun;Zhuo, Zhihang;Hu, Jiameng;Zhuo, Zhihang;Lu, Baoqian
关键词：Single-molecule sequencing; RNA-Seq; Transcriptome; Different developmental stage; Differentially expressed genes
-
Potential distribution of an invasive pest, Euplatypus parallelus, in China as predicted by Maxent
作者：Tang, Jihong;Lu, Hui;Lu, Fuping;Lu, Baoqian;Li, Jinhua
关键词：Euplatypus parallelus; maximum entropy; potential distribution area; invasive pest
-
Nutrient addition increases the capacity for division of labor and the benefits of clonal integration in an invasive plant
作者：Huang, Qiaoqiao;Li, Xiaoxia;Lu, Baoqian;Shen, Yide;Fan, Zhiwei;Lin, Peiqun;Huang, Fangfang;Wang, Ruilong
关键词：Clonal plant; Environmental heterogeneity; Mikania micrantha; Plant invasion; Root to shoot ratio
