Transcriptome and gene expression analysis of Rhynchophorus ferrugineus (Coleoptera: Curculionidae) during developmental stages
文献类型： 外文期刊
作者： Yang, Hongjun 1 ; Xu, Danping 1 ; Zhuo, Zhihang 1 ; Hu, Jiameng 2 ; Lu, Baoqian 4 ;
作者机构： 1.China West Normal Univ, Coll Life Sci, Nanchong, Sichuan, Peoples R China
2.Hainan Univ, Key Lab Genet & Germplasm Innovat Trop Special Fo, Key Lab Germplasm Resources Biol Trop Special Orn, Coll Forestry,Minist Educ, Haikou, Hainan, Peoples R China
3.South China Agr Univ, Key Lab Integrated Pest Management Crops South Ch, Minist Agr, Guangzhou, Guangdong, Peoples R China
4.Chinese Acad Trop Agr Sci, Key Lab Integrated Pest Management Trop Crops, Minist Agr China, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China
关键词： Single-molecule sequencing; RNA-Seq; Transcriptome; Different developmental stage; Differentially expressed genes
期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）
ISSN： 2167-8359
年卷期： 2020 年 8 卷
页码：
收录情况： SCI
摘要： Background. Red palm weevil, Rhynchophorus ferrugineus Olivier, is one of the most destructive pests harming palm trees. However, genomic resources for R. ferrugineus are still lacking, limiting the ability to discover molecular and genetic means of pest control. Methods. In this study, PacBio Iso-Seq and Illumina RNA-seq were used to generate transcriptome from three developmental stages of R. ferrugineus (pupa, 7th-instar larva, adult) to increase the understanding of the life cycle and molecular characteristics of the pest. Results. Sequencing generated 625,983,256 clean reads, from which 63,801 full-length transcripts were assembled with N50 of 3,547 bp. Expression analyses revealed 8,583 differentially expressed genes (DEGs). Moreover, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that these DEGs were mainly related to the peroxisome pathway which associated with metabolic pathways, material transportation and organ tissue formation. In summary, this work provides a valuable basis for further research on the growth and development, gene expression and gene prediction, and pest control of R. ferrugineus
- 相关文献
作者其他论文 更多>>
-
Control of postharvest stem-end rot on mango by antifungal metabolites of Trichoderma pinnatum LS029-3
作者：Zhan, Xin;Khan, Raja Asad Ali;Zhang, Jingya;Yin, Yaping;Wang, Rui;Liu, Tong;Chen, Jie;Yin, Yaping;Tang, Zhengbin;Lu, Baoqian;Lu, Baoqian;Liu, Tong
关键词：Lasiodiplodia theobromae; Botryodiplodia theobromae; fruit; non-volatile metabolites; GCMS; SEM
-
Characterization and analysis of the transcriptome in Opisina arenosella from different developmental stages using single-molecule real-time transcript sequencing and RNA-seq
作者：Xu, Danping;Zhuo, Zhihang;Yang, Hongjun;Zhuo, Zhihang;Hu, Jiameng;Yang, Fan;Lu, Baoqian
关键词：Single-molecule real-time sequencing; Illumina RNA sequencing; Opisina arenosella
-
SMRT sequencing of the full-length transcriptome of the Rhynchophorus ferrugineus (Coleoptera: Curculionida)
作者：Yang, Hongjun;Zhuo, Zhihang;Hu, Jiameng;Xu, Danping;Zhuo, Zhihang;Lu, Baoqian
关键词：Rhynchophorus ferrugineus; Different developmental stage; PacBio Iso-Seq; Illumina RNA-seq; Full-length transcriptome
-
Influence of climatic factors on the potential distribution of pest Heortia vitessoides Moore in China
作者：Xu, Danping;Li, Xinyang;Jin, Yawen;Zhuo, Zhihang;Yang, Hongjun;Hu, Jiameng;Xu, Danping;Xu, Danping;Zhuo, Zhihang;Wang, Rulin
关键词：Heortia vitessoides Moore; Potential distribution area; Climatic factors; MaxEnt modeling
-
Inter-country trade, genetic diversity and bio-ecological parameters upgrade pest risk maps for the coconut hispid Brontispa longissima
作者：Lu, Baoqian;Peng, Zhengqiang;Lu, Hui;Lin, Peiqun;Chu, Xiaoqiang;He, Xing;Tang, Jihong;Yang, Fan
关键词：invasive beetles; Brontispa longissima; global trade; biological trait; invasion biology
-
Potential distribution of an invasive pest, Euplatypus parallelus, in China as predicted by Maxent
作者：Tang, Jihong;Lu, Hui;Lu, Fuping;Lu, Baoqian;Li, Jinhua
关键词：Euplatypus parallelus; maximum entropy; potential distribution area; invasive pest
-
Nutrient addition increases the capacity for division of labor and the benefits of clonal integration in an invasive plant
作者：Huang, Qiaoqiao;Li, Xiaoxia;Lu, Baoqian;Shen, Yide;Fan, Zhiwei;Lin, Peiqun;Huang, Fangfang;Wang, Ruilong
关键词：Clonal plant; Environmental heterogeneity; Mikania micrantha; Plant invasion; Root to shoot ratio
