文献类型： 外文期刊

作者： Song, Yanbo ¹ ; Shi, Xiaojing ² ; Zou, Yanli ² ; Guo, Juanru ² ; Huo, Nan ² ; Chen, Shuangjian ³ ; Zhao, Chengping ² ; Li ¹ ;

作者机构： 1.Shanxi Agr Univ, Hort Coll, Taigu 030801, Shanxi, Peoples R China

2.Shanxi Agr Univ, Life Sci Coll, Taigu 030801, Shanxi, Peoples R China

3.Shanxi Acad Agr Sci, Inst Pomol, Taigu 030801, Shanxi, Peoples R China

4.Shanghai Appl Prot Technol Co Ltd, Shanghai 201100, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2020 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Ubiquitination is a critical post-translational modification machinery that governs a wide range of cellular functions by regulating protein homeostasis. Identification of ubiquitinated proteins and lysine residues can help researchers better understand the physiological roles of ubiquitin modification in different biological systems. In this study, we report the first comprehensive analysis of the peach ubiquitome by liquid chromatography-tandem mass spectrometry-based diglycine remnant affinity proteomics. Our systematic profiling revealed a total of 544 ubiquitination sites on a total of 352 protein substrates. Protein annotation and functional analysis suggested that ubiquitination is involved in modulating a variety of essential cellular and physiological processes in peach, including but not limited to carbon metabolism, histone assembly, translation and vesicular trafficking. Our results could facilitate future studies on how ubiquitination regulates the agricultural traits of different peach cultivars and other crop species.