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Rapid generation of a tomato male sterility system and its feasible application in hybrid seed production

文献类型： 外文期刊

作者： Zhou, Ming 1 ; Deng, Lei 2 ; Yuan, Guoliang 1 ; Zhao, Wei 2 ; Ma, Mingyang 1 ; Sun, Chuanlong 2 ; Du, Minmin 3 ; Li, Chuanyou 2 ; Li, Changbao 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Inst Vegetable Sci, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China

2.Chinese Acad Sci, Innovat Acad Seed Design, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol,State Key Lab Plant Genom, Beijing 100101, Peoples R China

3.China Agr Univ, Coll Hort, Beijing Key Lab Growth & Dev Regulat Protected Veg, Beijing 100193, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 9 期

页码：

收录情况： SCI

摘要： Key messageA practical approach for the rapid generation and feasible application of green hypocotyl male-sterile (GHMS) tm6 dfr lines in tomato hybrid breeding was established.AbstractMale sterility enables reduced cost and high seed purity during hybrid seed production. However, progress toward its commercial application has been slow in tomato due to the disadvantages of most natural male-sterile mutants. Here, we developed a practical method for efficient tomato hybrid seed production using a male-sterile system with visible marker, which was rapidly generated by CRISPR/Cas9-mediated gene editing. Two closely linked genes, TM6 and DFR, which were reported to be candidates of ms15 (male sterile-15) and aw (anthocyanin without) locus, respectively, were knocked out simultaneously in two elite tomato inbred lines. Mutagenesis of both genes generated green hypocotyl male-sterile (GHMS) lines. The GHMS lines exhibited male sterility across different genetic backgrounds and environmental conditions. They also showed green hypocotyl due to defective anthocyanin accumulation, which serves as a reliable visible marker for selecting male-sterile plants at the seedling stage. We further proposed a strategy for multiplying the GHMS system and verified its high efficiency in stable male sterility propagation. Moreover, elite hybrid seeds were produced using GHMS system for potential side effects evaluation, and no adverse influences were found on seed yield, seed quality as well as important agronomic traits. This study provides a practical approach for the rapid generation and feasible application of male sterility in tomato hybrid breeding.

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