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Metabolic and transcriptomic analysis of two Cucurbita moschata germplasms throughout fruit development

文献类型: 外文期刊

作者: Abbas, Hafiz Muhammad Khalid 1 ; Huang, He-Xun 1 ; Wang, An-Jun 1 ; Wu, Ting-Quan 1 ; Xue, Shu-Dan 1 ; Ahmad, Aqeel 1 ;

作者机构: 1.Guangdong Acad Agr Sci, Guangdong Key Lab New Technol Res Vegetables, Vegetable Res Inst, Guangzhou 510640, Peoples R China

关键词: Cucurbita moschata; Carotenoids; Sugars; Organic acids; Transcriptome

期刊名称:BMC GENOMICS ( 影响因子:3.969; 五年影响因子:4.478 )

ISSN: 1471-2164

年卷期: 2020 年 21 卷 1 期

页码:

收录情况: SCI

摘要: Background Pumpkins (Cucurbita moschata; Cucurbitaceae) are valued for their fruits and seeds and are rich in nutrients. Carotenoids and sugar contents, as main feature of pumpkin pulp, are used to determine the fruit quality. Results Two pumpkin germplasms, CMO-X and CMO-E, were analyzed regarding the essential quality traits such as dry weight, soluble solids, organic acids, carotenoids and sugar contents. For the comparison of fruit development in these two germplasms, fruit transcriptome was analyzed at 5 different developmental stages from 0 d to 40 d in a time course manner. Putative pathways for carotenoids biosynthesis and sucrose metabolism were developed in C. moschata fruit and homologs were identified for each key gene involved in the pathways. Gene expression data was found consistent with the accumulation of metabolites across developmental stages and also between two germplasms. PSY, PDS, ZEP, CRTISO and SUS, SPS, HK, FK were found highly correlated with the accumulation of carotenoids and sucrose metabolites, respectively, at different growth stages of C. moschata as shown by whole transcriptomic analysis. The results of qRT-PCR analysis further confirmed the association of these genes. Conclusion Developmental regulation of the genes associated with the metabolite accumulation can be considered as an important factor for the determination of C. moschata fruit quality. This research will facilitate the investigation of metabolic profiles in other cultivars.

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