The application of a novel biomimetic enzyme p-BEs cascade catalytic platform for the rapid detection of glucose
文献类型： 外文期刊
作者： Gu, Long 1 ; Zeng, Sili 1 ; Fan, Ze 1 ; Qian, Wenqi 1 ; Qin, Dongli 1 ; Chen, Zhongxiang 1 ; Huang, Li 1 ; Bai, Shuyan 1 ; Xie, Haijiao 6 ; Gao, Lei 1 ; Wang, Peng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China
2.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.Minist Agr & Rural Affairs, Supervis Inspect & Testing Ctr Fishery Environm &, Harbin 150070, Peoples R China
5.Minist Agr & Rural Affairs, Key Lab Control Qual & Safety Aquat Prod, Beijing 100141, Peoples R China
6.Hangzhou Yanqu Informat Technol Co Ltd, Y2,2nd Floor,Bldg 2,Xixi Legu Creat Pioneering Pk,, Hangzhou 310003, Zhejiang, Peoples R China
关键词： Visual detection; Rapid detection; Novel biomimetic enzyme
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 2023影响因子：7.7； 五年影响因子：7.7 ）
ISSN： 0141-8130
年卷期： 2024 年 277 卷
收录情况： SCI
摘要： The blood glucose concentration in aquatic organisms, a crucial indicator reflecting their health status, holds significant importance for detecting glucose levels in serum in terms of processing and quality monitoring. In this study, a novel POD biomimetic enzyme (p-BEs) with horseradish peroxidase catalytic properties was designed, optimized, and its mechanism was discussed in detail. Based on this, a portable system has been developed capable of determining glucose levels in three ways: quantitatively analyzed through UV-Vis/MD, quantitatively analyzed on-site using a mobile phone RGB, and semi-quantitatively analyzed through a drip plate. Meanwhile, compared with other catalytic methods for detecting glucose, we achieved a lower limit of detection (0.03 mu M) and shorter detection time (12 min), with high catalytic activity. This study provides new insights into the design of efficient and reliable cascade catalytic systems responsive to glucose, offering a low-cost, simplicity of operation method for glucose detection.
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