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Narrowing Down a Major QTL Region Conferring Pod Fiber Contents in Yardlong Bean (Vigna unguiculata), a Vegetable Cowpea

文献类型： 外文期刊

作者： Watcharatpong, Phurisorn 1 ; Kaga, Akito 2 ; Chen, Xin 3 ; Somta, Prakit 1 ;

作者机构： 1.Kasetsart Univ, Fac Agr Kamphaeng Saen, Dept Agron, Kamphaeng Saen Campus, Kamphaeng Saen 73140, Nakhon Pathom, Thailand

2.Natl Agr & Food Res Org, Soybean & Field Crop Appl Genom Res Unit, Inst Crop Sci, 2-1-2 Kannondai, Tsukuba, Ibaraki 3058602, Japan

3.Jiangsu Acad Agr Sci, Inst Ind Crops, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China

4.Ctr Excellence Agr Biotechnol AG BIO PERDO CHE, Bangkok 10900, Thailand

关键词： pod fibers; cellulose; hemicellulos; lignin; pod shattering; beta glucosidase; MYB26

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN：

年卷期： 2020 年 11 卷 4 期

页码：

收录情况： SCI

摘要： Yardlong bean (Vigna unguiculata (L.) Walp. ssp. sesquipedalis), a subgroup of cowpea, is an important vegetable legume crop of Asia where its young pods are consumed in both fresh and cooked forms. Pod fiber contents (cellulose, hemicellulose and lignin) correlates with pod tenderness (softness/hardness) and pod shattering. In a previous study using populations derived from crosses between yardlong bean and wild cowpea (V. unguiculata ssp. unguiculata var. spontanea), three major quantitative trait loci (QTLs), qCel7.1, qHem7.1 and qLig7.1, controlling these fibers were identified on linkage group 7 (cowpea chromosome 5) and are co-located with QTLs for pod tenderness and pod shattering. The objective of this study was to identify candidate gene(s) controlling the pod fiber contents. Fine mapping for qCel7.1, qHem7.1 and qLig7.1 was conducted using F-2 and F-2:3 populations of 309 and 334 individuals, respectively, from the same cross combination. New DNA markers were developed from cowpea reference genome sequence and used for fine mapping. A QTL analysis showed that in most cases, each pod fiber content was controlled by one major and one minor QTLs on the LG7. The major QTLs for cellulose, hemicellulose and lignin in pod were always mapped to the same regions or close to each other. In addition, a major QTL for pod shattering was also located in the region. Although there were several annotated genes relating to pod fiber contents in the region, two genes including Vigun05g266600 (VuBGLU12) encoding a beta glucosidase and Vigun05g273500 (VuMYB26b) encoding a transcription factor MYB26 were identified as candidate genes for the pod fiber contents and pod shattering. Function(s) of these genes in relation to pod wall fiber biosynthesis and pod shattering was discussed.

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