文献类型： 外文期刊
作者： Tong, Bin 1 ; Wang, Jiapeng 1 ; Cheng, Zixuan 1 ; Liu, Jiasen 2 ; Wu, Yiran 3 ; Li, Yunhua 2 ; Bai, Chunling 1 ; Zhao, Su 1 ;
作者机构： 1.Inner Mongolia Univ, Res Ctr Lab Anim Sci, Sch Life Sci, State Key Lab Reprod Regulat & Breeding Grassland, Hohhot 010070, Peoples R China
2.Inner Mongolia Acad Agr & Anim Husb Sci, Inst Anim Sci, Hohhot 010018, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, iHuman Inst, Shanghai 201210, Peoples R China
关键词： FecB(B); GDF9; litter size; Mongolia sheep; promoter activity; molecular markers
期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）
ISSN：
年卷期： 2020 年 11 卷 4 期
页码：
收录情况： SCI
摘要： Litter size is an economically important trait in sheep breeding. The objectives of this study were as follows: (1) to ascertain if any of the 19 known variants in the BMPRIB, BMP15, and GDF9 genes are present and associated with the litter size of Mongolia sheep; (2) to identify novel variants in GDF9 and perform association analysis; and (3) to validate the effects of these GDF9 promoter variants on the activity of the gene. The results of the 19 known variants showed that the FecB(B) affected the litter size of Mongolia sheep (p < 0.001). The association analysis results of novel variants showed that the g.46544883A>G (GenBank accession: NC_040256, the same below) in the 3' untranslated region (3' UTR), the c.1040T>C (Phe347Ser) in the exon 2, and the g.46547859C>T SNP in the promotor of GDF9 were significantly associated with litter size of Mongolia ewes (p < 0.01, p < 0.05, and p < 0.001, respectively). In addition, the GDF9 promoter activity analysis showed that the C allele at the -332 position (g.46547859C>T) could decrease luciferase activity compared with the T allele (p < 0.01). Our findings may facilitate effective marker-assisted selection to increase litter size in Mongolia sheep populations, as well as bring new insights into GDF9 expression.
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