CsSNAT positively regulates salt tolerance and growth of cucumber by promoting melatonin biosynthesis
文献类型： 外文期刊
作者： Qi, Chuandong 1 ; Zhang, Haijun 2 ; Liu, Ying 1 ; Wang, Xiaoyun 1 ; Dong, Danhui 1 ; Yuan, Xiaowei 3 ; Li, Xingsheng 3 ;
作者机构： 1.China Agr Univ, Coll Hort, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100193, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
3.Shandong Huasheng Agr Co Ltd, Shandong Prov Key Lab Cucurbitaceae Vegetable Bio, Shandong 262500, Peoples R China
4.Beijing Univ Agr, Coll Plant Sci & Technol, Beijing 102206, Peoples R China
关键词： CsSNAT; Cucumber (Cucumis sativus); Growth; Melatonin; ROS accumulation; Salt stress
期刊名称：ENVIRONMENTAL AND EXPERIMENTAL BOTANY （ 2020影响因子：5.545； 五年影响因子：5.99 ）
ISSN： 0098-8472
年卷期： 2020 年 175 卷
收录情况： SCI
摘要： Salt stress is an important limiting factor in agricultural production. Previous studies have shown that exogenous melatonin (N-acetyl-5-methoxytryptamine) promotes plant defense against various abiotic stresses in a concentration dependent manner. It is an efficient and convenient way to improve the resistance of plants by modulating the endogenous melatonin at a moderately high level by utilizing the plant's own regulatory mechanism. Serotonin N-acetyltransferase (SNAT) is a key enzyme in the melatonin synthesis pathway. In this study, CsSNAT overexpression lines and CsSNAT RNA interference (RNAi) lines were generated. The content of endogenous melatonin increased 2-3-fold in overexpression lines (OE-15 and OE-40) and decreased 0.5-0.6-fold in RNAi lines (RNAi-5 and RNAi-7). After three days of treatment with 100 mM NaCl, the overexpression lines exhibited stronger salt tolerance compared to wild type and RNAi lines. The malondialdehyde (MDA) content, relative electrical conductivity (REC), proline content, maximum photochemical efficiency of photosystem II (Fv/Fm), reactive oxygen species (ROS) accumulation, and the activities of antioxidant enzymes showed more significant salt stress injury in the RNAi lines. Under salt stress, there was significantly higher expression of stress resistance-related genes such as CsSOD, CsCAT, CsP5CS1, CsP5CS2, CsPIP, and CsHSP90 in the overexpression lines compared to the wild type and the RNAi lines. Further analysis revealed that overexpression of the CsSNAT gene in cucumber promoted seed germination. The growth of cucumber seedlings of the RNAi lines was severely inhibited. Taken together, our results suggest that CsSNAT positively regulates salt tolerance and growth of cucumber seedlings by promoting melatonin synthesis.
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