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Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis

文献类型： 外文期刊

作者： Quan, Rong 1 ; Wei, Li 1 ; Hou, Lei 1 ; Wang, Jing 1 ; Zhu, Shanshan 1 ; Li, Zixuan 1 ; Lv, Moran 1 ; Liu, Jue 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Prevent & Control Infect Dis Live, Inst Anim Husb & Vet Med, 9 Shuguang Garden Middle Rd, Beijing 100097, Peoples R China

关键词： aMPV/C; differentially expressed proteins; iTRAQ; PLK2; apoptosis

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN：

年卷期： 2020 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Avian metapneumovirus subtype C (aMPV/C) causes an acute respiratory disease that has caused serious economic losses in the Chinese poultry industry. In the present study, we first explored the protein profile in aMPV/C-infected Vero cells using iTRAQ quantitative proteomics. A total of 921 of 7034 proteins were identified as significantly altered by aMPV/C infection. Three selected proteins were confirmed by Western blot analysis. Bioinformatics GO analysis revealed multiple signaling pathways involving cell cycle, endocytosis, and PI3K-Akt, mTOR, MAPK and p53 signaling pathways, which might participate in viral infection. In this analysis, we found that PLK2 expression was upregulated by aMPV/C infection and investigated whether it contributed to aMPV/C-mediated cellular dysfunction. Suppressing PLK2 attenuated aMPV/C-induced reactive oxygen species (ROS) production and p53-dependent apoptosis and reduced virus release. These results in a mammalian cell line suggest that high PLK2 expression correlates with aMPV/C-induced apoptosis and viral replication, providing new insight into the potential avian host cellular response to aMPV/C infection and antiviral targets.

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