Rapid detection of two emerging viruses associated with necrotic symptoms in Areca catechu L. by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
文献类型： 外文期刊
作者： Zhao, Guangyuan 1 ; Shen, Wentao 2 ; Tuo, Decai 2 ; Cui, Hongguang 1 ; Yan, Pu 2 ; Tang, Qinghua 4 ; Zhu, Guopeng 1 ; Li, 1 ;
作者机构： 1.Hainan Univ, Inst Trop Agr & Forestry, Haikou 570228, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China
3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
4.Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Peoples R China
关键词： RT-LAMP; ANRSV; ANSSV; Areca palm
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
ISSN： 0166-0934
年卷期： 2020 年 281 卷
页码：
收录情况： SCI
摘要： Two reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of areca palm necrotic ringspot virus (ANRSV) and areca palm necrotic spindle-spot virus (ANSSV), respectively. These two emerging viruses both induce necrotic symptoms in areca palms. The coat protein (CP) gene of ANRSV and the 9 K gene of ANSSV were used to design the respective RT-LAMP primers for the assays. Each set of four primers designed for each of these viruses was found to be highly specific in the detection of the respective targeted virus. The optimal incubation conditions for the RT-LAMP assays were 63 degrees C for 40 min for ANRSV and a 61 degrees C for 40 min for ANSSV. The sensitivity of the RT-LAMP method for each of these viruses was 10-fold greater than that of the corresponding conventional reverse-transcription polymerase chain reaction (RT-PCR). The RT-LAMP assays may be useful for the rapid early detection of ANSSV and ANRSV in commercial areca palm production.
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