Synergistic mutations of two rapeseed AHAS genes confer high resistance to sulfonylurea herbicides for weed control
文献类型： 外文期刊
作者： Guo, Yue 1 ; Cheng, Li 1 ; Long, Weihua 1 ; Gao, Jianqin 1 ; Zhang, Jiefu 1 ; Chen, Song 1 ; Pu, Huiming 1 ; Hu, Maolong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Ind Crops, Natl Ctr Oil Crops Improvement,Nanjing Subctr, Key Lab Cotton & Rapeseed Nanjing,Minist Agr, Nanjing 210014, Peoples R China
2.Jiangsu Univ, Inst Life Sci, 301 Xuefu Rd, Zhenjiang 212013, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Peoples R China
期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）
ISSN： 0040-5752
年卷期： 2020 年 133 卷 10 期
页码：
收录情况： SCI
摘要： Key message A double mutant 5N of rapeseed was obtained with a synergistic effect of high resistance to sulfonylurea herbicide. Excellent weed control was observed in Ning R201 created by 5N resources. Sulfonylurea herbicides, which inhibit acetohydroxyacid synthase (AHAS), have become the most widely used herbicides worldwide. However, weed control in rapeseed crop production remains challenging in China due to the shortage of available herbicide-resistant cultivars. In this study, we developed a rapeseed line (PN19) with sulfonylurea herbicide resistance through seed mutagenesis. Molecular analysis revealed a Trp-574-Leu mutation inBnAHAS1-2Rof PN19 according to the sequence ofArabidopsis thaliana, and an allele-specific cleaved amplified polymorphic sequence marker was developed to target the point mutation. A double mutant (5N) with very high sulfonylurea resistance was then created through pyramiding two mutant genes of PN19 and M342 by molecular marker-assisted selection. Herbicide resistance identification, toxicology testing, and an in vitro enzyme activity assay of AHAS in 5N indicated that each mutant was four and eight times more resistant to sulfonylurea than M342 and PN19, respectively. Protein structure analysis of AHAS1 demonstrated that the leucine of mutant Trp-574-Leu destroyed the original pi-plane stacking effect of the local region for tribenuron-methyl binding, leading to herbicide tolerance. Isobole graph analysis showed a significant synergistic effect of the combination of two mutant genes in 5N for improved tolerance to sulfonylurea herbicides. Finally, we bred rapeseed variety Ning R201 using 5N herbicide resistance resources, and observed excellent weed control performance. Together, these results demonstrate the practical value of 5N application for optimizing and simplifying rapeseed cultivation in China.
- 相关文献
作者其他论文 更多>>
-
Comparative genome-wide analysis of circular RNAs in Brassica napus L.: target-site versus non-target-site resistance to herbicide stress
作者：Guo, Yue;Wang, Ting;Lu, Xinyu;Li, Weilong;Lv, Xinlei;Peng, Qi;Zhang, Jiefu;Gao, Jianqin;Hu, Maolong
关键词：
-
Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis
作者：Sun, Chengming;Zhou, Xiaoying;Fu, Sanxiong;Wang, Xiaodong;Peng, Qi;Gao, Jianqin;Chen, Feng;Zhang, Wei;Hu, Maolong;Zhang, Jiefu;Wu, Jian;Liu, Huimin;Wang, Youping;Xue, Zhifei;Fu, Tingdong;Yi, Bin
关键词：Rapeseed; Branch angle; Homoeologous exchange; WGCNA; QTL-seq; WRKY40
-
Identification of genetic loci for salt tolerance in Brassica napus at the germination stage using a genome-wide association study
作者：Yang, Tinghai;Zhu, Bin;Wang, Xiaodong;Yang, Tinghai;Zhou, Xiaoying;Cao, Yu;Shi, Rui;Wang, Yaping;Xiang, Yanan;Chen, Feng;Zhang, Wei;Sun, Chengming;Fu, Sanxiong;Hu, Maolong;Zhang, Jiefu;Wang, Xiaodong;Cao, Yu;Chen, Feng;Fu, Sanxiong;Hu, Maolong;Zhang, Jiefu;Wang, Xiaodong
关键词：Brassica napus; Genome-wide association study; Salt tolerance; SNP molecular marker; Candidate genes; Germplasm resources
-
Genome-Wide Association Study Reveals the Genetic Basis of Crude Fiber Components in Brassica napus L. Shoots at Stem Elongation Stage
作者：Shi, Rui;Cao, Yu;Yang, Tinghai;Wang, Yaping;Xiang, Yanan;Chen, Feng;Zhang, Wei;Zhou, Xiaoying;Sun, Chengming;Fu, Sanxiong;Hu, Maolong;Zhang, Jiefu;Wang, Xiaodong;Shi, Rui;Cao, Yu;Fu, Sanxiong;Hu, Maolong;Zhang, Jiefu;Wang, Xiaodong
关键词：Brassica napus; shoots; crudefiber components; GWAS; candidate gene; germplasm resource
-
Comparative Transcriptome Analysis Reveals the Molecular Basis of Brassica napus in Response to Aphid Stress
作者：Li, Yuanhong;Cai, Lei;Ding, Ting;Tian, Entang;Yu, Kunjiang;Cai, Lei;Yu, Kunjiang;Chen, Zhuo;Yan, Xiaohong;Wang, Xiaodong;Zhang, Jiefu;Yu, Kunjiang
关键词：Brassica napus; resistance to aphid stress; transcriptome characteristics; physiological characteristics; candidate gene
-
Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed Rape
作者：Peng, Qi;Zhou, Xiaoying;Sun, Chengming;Hu, Maolong;Fu, Sanxiong;Zhang, Jiefu;Peng, Qi;Li, Wei;Hou, Yan;Lei, Lei;Kundu, Jiban Kumar;Kundu, Jiban Kumar
关键词：BrYV; TuYV; oilseed rape; phylogenetic tree; incidence; leaf color
-
Quantitative Trait Locus Mapping Combined with RNA Sequencing Reveals Candidate Genes for Chlorophyll Content in Oilseed Rape Leaves
作者：Li, Yuanhong;Cai, Lei;He, Yuqi;Ding, Ting;Tian, Entang;Yu, Kunjiang;Yu, Kunjiang;Zhuo, Chen;Yu, Kunjiang;Zhang, Jiefu;Wang, Xiaodong
关键词：Brassica napus; Quantitative trait loci; Transcriptome; Chlorophyll content
