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Fine-mapping and candidate gene analysis of qFS-Chr. D02, a QTL for fibre strength introgressed from a semi-wild cotton into Gossypium hirsutum

文献类型： 外文期刊

作者： Feng, Liuchun 1 ; Zhou, Chenhui 1 ; Su, Qiao 1 ; Xu, Min 1 ; Yue, Haoran 1 ; Zhang, Shuwen 1 ; Zhou, Baoliang 1 ;

作者机构： 1.Nanjing Agr Univ, MOE Hybrid Cotton R&D Engn Res Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Fine mapping; Fibre strength (FS); Gossypium hirsutum; Introgression line (IL); Quantitative trait loci (QTLs); qRT-PCR

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2020 年 297 卷

页码：

收录情况： SCI

摘要： Fibre strength (FS) is an important quality attribute in the modern textile industry, which is genetically controlled by quantitative trait loci (QTLs). Fine-mapping stable QTLs for FS to identify candidate genes would be valuable for uncovering the genetic basis of fibre quality traits in cotton. Here, a single segment introgression line, IL-D2 - 2, from the cross of (TM-1 x TX-1046) reported in our previous studies, was found to have significantly improved FS compared with the recurrent parent TM-1. To fine-map the QTLs of the FS, we further crossed IL-D2 - 2 with its recurrent parent TM-1 to produce F-2 and F-2.3 populations. QTL analysis and substitution mapping showed qFS-Chr. D02 was anchored into a 550.66 kb-interval between two markers, INTR1027 and JESPR-231. This interval contained 67 genes, among which 27 genes related to cell-wall synthesis were selected to conduct qRT-PCR. The results revealed seven genes were expressed significantly differently during the fibre secondary-wall-thickening stage (10-25 days post-anthesis), three being upregulated and four downregulated in IL-D2 - 2. Both GH_D02G2269 (UDP-glucosyl transferase 84B1) and GH_D02G2289 (unknown function (DUF869)) with nonsynonymous SNPs in IL-D2 - 2 had significantly downregulated expression, suggesting they were candidates for qFS-Chr. D02. This research provides information about marker-assisted selection for cotton fibre strength improvement.

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