文献类型： 外文期刊
作者： Wu, Qingfei 1 ; Zhang, Cheng 1 ; Chen, Yue 2 ; Zhou, Kaiyue 1 ; Zhan, Yihua 1 ; Jiang, Dean 1 ;
作者机构： 1.Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China
关键词： rice; OsCpn60 beta 1; chloroplast; proteomics
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2020 年 21 卷 11 期
页码：
收录情况： SCI
摘要： The chaperonin 60 (Cpn60) protein is of great importance to plants due to its involvement in modulating the folding of numerous chloroplast protein polypeptides. In chloroplasts, Cpn60 is differentiated into two subunit types-Cpn60 alpha and Cpn60 beta and the rice genome encodes three alpha and three beta plastid chaperonin subunits. However, the functions of Cpn60 family members in rice were poorly understood. In order to investigate the molecular mechanism of OsCpn60 beta 1, we attempted to disrupt the OsCpn60 beta 1 gene by CRISPR/Cas9-mediated targeted mutagenesis in this study. We succeeded in the production of homozygous OsCpn60 beta 1 knockout rice plants. The OsCpn60 beta 1 mutant displayed a striking albino leaf phenotype and was seedling lethal. Electron microscopy observation demonstrated that chloroplasts were severely disrupted in the OsCpn60 beta 1 mutant. In addition, OsCpn60 beta 1 was located in the chloroplast and OsCpn60 beta 1 is constitutively expressed in various tissues particularly in the green tissues. The label-free qualitative proteomics showed that photosynthesis-related pathways and ribosomal pathways were significantly inhibited in OsCpn60 beta 1 mutants. These results indicate that OsCpn60 beta 1 is essential for chloroplast development in rice.
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