Extracellular Nanovesicle Enhanced Gene Transfection Using Polyethyleneimine in HEK293T Cells and Zebrafish Embryos
文献类型： 外文期刊
作者： Zhang, Zhenzhen 1 ; Wen, Kai 1 ; Zhang, Chao 3 ; Laroche, Fabrice 4 ; Wang, Zhenglong 1 ; Zhou, Qiang 5 ; Liu, Zunfeng 6 ;
作者机构： 1.China Pharmaceut Univ, Dept Sci, Nanjing, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Peoples R China
3.China Pharmaceut Univ, Sch Tradit Chinese Pharm, Nanjing, Peoples R China
4.Aarhus Univ, Dept Mol Biol & Genet, Ctr Carbohydrate Recognit & Signalling, Aarhus, Denmark
5.Nankai Univ, Tianjin First Cent Hosp, Dept Orthopaed, Tianjin, Peoples R China
6.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Key Lab Funct Polymer Mat, Tianjin, Peoples R China
7.Univ Basel, C CINA, Biozentrum, Basel, Switzerland
关键词： gene transfection; extracellular nanovesicles; zebra fish; polyethyleneimine; efficiency
期刊名称：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY （ 2020影响因子：5.89； 五年影响因子：5.973 ）
ISSN： 2296-4185
年卷期： 2020 年 8 卷
收录情况： SCI
摘要： It is a hot topic to improve efficiency and decrease toxicity of gene transfection reagents. The extracellular nanovesicles (EVs) that are released by cells play an important role in intercellular communication and are naturally designed for genetic exchange between cells. Here, we show that the EVs have a large beneficial effect in polyethyleneimine (PEI)-mediated transfection of a GFP-encoding plasmid into HEK293T cells. An improvement of transfection efficiency of similar to 500% and a decrease in toxicity were observed in a specific concentration range of PEI. The EVs also greatly improved the transfection of the same plasmid into zebrafish embryos. To verify the generality of this gene transfection approach, we also tested the cell viability and gene transfection efficiency using two other plasmids (EpTEN and ELuc) and in another cell line (A549). The measured increase in transfection efficiency makes EV a promising candidate for enhancement of the quality of current PEI-based transfection technique.
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