文献类型： 外文期刊

作者： Huo Huanhuan ¹ ; Gao Xiaoqiang ¹ ; Fei Fan ¹ ; Qin Fei ¹ ; Huang Bin ¹ ; Liu Baoliang ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China

3.Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Jiangxi, Peoples R China

关键词： turbot; transcriptome; crowding stress; immune response

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：0.913； 五年影响因子：1.012 ）

ISSN： 1672-5182

年卷期： 2020 年 19 卷 4 期

页码：

收录情况： SCI

摘要： In this study, juvenile turbotScophthalmus maximuswere vaccinated with attenuatedEdwardsiella tarda(EIBAV1) and reared at two different densities, low density (LD), (5.25 +/- 0.02)kgm(-2), as control group and high density (HD), (20.53 +/- 0.05) kg m(-2), as experimental group. Only density was considered as the variable. Five weeks after vaccination, the transcriptomes of spleen and head kidney from the turbot in two groups were analyzed with RNA-Seq technology. A total of 447 million reads were assembled into 41136 genes with an average length of 1274 bp and a N(50)size of 2295 bp. A comparison of gene expression between HD and LD groups revealed 1155 differentially expressed genes (DEGs). Enrichment and pathway analysis of the 10 immune-related DEGs showed the centrality of toll-like receptor signaling pathway, cytosolic DNA-sensing pathway and platelet activation in the host immune responses. The 5 overexpressed inflammatory cytokines and 5 downregulated signal-regulated cytokines genes are covered by these immune-related DEGs. It was inferred that cells suffer damage and the immune response is restrained in turbot under crowding stress.