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Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptideCaAMP1gene in soybean

文献类型： 外文期刊

作者： Niu, Lu 1 ; Zhong, Xiaofang 1 ; Zhang, Yuanyu 1 ; Yang, Jing 1 ; Xing, Guojie 1 ; Li, Haiyun 1 ; Liu, Dongbo 1 ; Ma, Rui; 1 ;

作者机构： 1.Jilin Acad Agr Sci, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China

2.Jilin Normal Univ, Siping 136000, Peoples R China

关键词： CaAMP1; Transgenic soybean; PRR tolerance; Antimicrobial peptide

期刊名称：BMC GENETICS （ 影响因子：2.797； 五年影响因子：3.263 ）

ISSN： 1471-2156

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Antimicrobial peptides play important roles in both plant and animal defense systems. Moreover, over-expression ofCaAMP1(Capsicum annuum antimicrobial protein 1), an antimicrobial protein gene isolated fromC. annuumleaves infected withXanthomonas campestrispv.vesicatoria, confers broad-spectrum resistance to hemibiotrophic bacterial and necrotrophic fungal pathogens inArabidopsis. Phytophthora root and stem rot (PRR), caused by the fungusPhytophthora sojae, is one of the most devastating diseases affecting soybean (Glycine max) production worldwide. Results In this study,CaAMP1was transformed into soybean byAgrobacterium-mediated genetic transformation. Integration of the foreign gene in the genome of transgenic soybean plants and its expression at the translation level were verified by Southern and western blot analyses, respectively.CaAMP1over-expression (CaAMP1-OX) lines inoculated withP. sojaerace 1 exhibited enhanced and stable PRR tolerance through T-2-T(4)generations compared with the wild-type Williams 82 plants. Gene expression analyses in the transgenic plants revealed that the expression of salicylic acid-dependent, jasmonic acid-dependent, and plant disease resistance genes (R-genes) were significantly up-regulated afterP. sojaeinoculation. Conclusions These results indicate thatCaAMP1over-expression can significantly enhance PRR tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways. This provides an alternative approach for developing soybean varieties with improved tolerance against soil-borne pathogenic PRR.

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