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Overexpression of the Sorghum bicolor K+/Na+ Transporter Gene, SbSKC1, Enhances Salt Tolerance in Poplar (Populus tomentosa)

文献类型： 外文期刊

作者： Yao, Xinzhuan 1 ; Chen, Mingjun 2 ; Zhao, Degang 2 ; Lv, Litang 1 ;

作者机构： 1.Guizhou Univ, Coll Tea Sci, Guiyang 550025, Peoples R China

2.Guizhou Univ, Inst Agrobioengn, Minist Educ, Coll Life Sci, Guiyang 550025, Peoples R China

3.Guizhou Univ, Inst Agrobioengn, Minist Educ, Key Lab Plant Resources Conservat & Germplasm Inn, Guiyang 550025, Peoples R China

4.Guizhou Acad Agr Sci, Guiyang 550006, Peoples R China

关键词： Poplar; SbSKC1 gene; Plant biomass; K+/Na+ ratio; Antioxidant enzyme; RT-PCR

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2020 年 24 卷 2 期

页码：

收录情况： SCI

摘要： The SbSKC1 gene is instrumental in plant salt stress response. In the current study, the SbSKC1 gene from Sorghum bicolor was overexpressed in poplar, using Agrobacterium-mediated genetic transformation, to study the effects of SbSKC1 on poplar salt tolerance. Differences in salt tolerance between wild type and transgenic poplar were compared by determining phenotypic and physiological indexes for the two strains. Under normal growth conditions, wild type and transgenic plants exhibited normal growth with no significant differences between the two strains. However, upon exposure to 150 mM salt stress, transgenic poplar grew significantly better than wild type, and wild type plants experienced more severe leaf blight than the transgenic plants. At the same time, the chlorophyll content of the transgene changed significantly more than the wild type. Root elongation is obvious, but biomass is not significant. In addition, transgenic plants maintained a high K+/Na+ ratio. Protective enzyme activities were enhanced in transgenic plants after salt stress. Alterations in malondialdehyde (MDA) levels indicated that transgenic seedlings developed less membrane injury during salt stress. When expression levels of genes related to salt-tolerance were compared in transgenic versus wild-type poplar plants, the results showed that transgenic plants increased expression levels for ERF76, SOD1, LEA1, P450, and Laccasel genes. We conclude that SbSKC1 confers salt stress tolerance by maintaining an elevated K+/Na+ ratio, suppressing membrane damage through enhanced activity of the antioxidant system, and increasing expression of genes related to salt tolerance. (C) 2020 Friends Science Publishers

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