文献类型： 外文期刊
作者： Zheng, Mingmin 1 ; Yang, Tian 1 ; Liu, Xiaowei 1 ; Lu, Guihua 2 ; Zhang, Peng 1 ; Jiang, Bin 1 ; Zhou, Shufeng 1 ; Lu, Ya 1 ;
作者机构： 1.Sichuan Agr Univ, Maize Res Inst, 211 Huimin Rd Wenjiang Dist, Chengdu 611130, Peoples R China
2.Zhejiang Acad Agr Sci, Dongyang Maize Res Inst Zhejiang Prov, Dongyang 322100, Peoples R China
关键词： Maize; Cytoplasmic male sterility; Fertility restoration; Restorer gene; QTL-seq
期刊名称：G3-GENES GENOMES GENETICS （ 影响因子：3.154； 五年影响因子：3.369 ）
ISSN： 2160-1836
年卷期： 2020 年 10 卷 7 期
页码：
收录情况： SCI
摘要： C-type cytoplasmic male sterility (CMS-C), one of the three major CMS types in maize, has a promising application prospect in hybrid seed production. However, the complex genetic mechanism underlying the fertility restoration of CMS-C remains poorly understood. The maize inbred line A619 is one of the rare strong restorer lines carrying the restorer geneRf4, but different fertility segregation ratios are found in several F(2)populations derived from crosses between isocytoplasmic allonucleus CMS-C lines and A619. In the present study, the segregation ratios of fertile to sterile plants in the (CHuangzaosi x A619) F(2)and BC(1)F(1)populations (36.77:1 and 2.36:1, respectively) did not follow a typical monogenic model of inheritance, which suggested that some F(2)and BC(1)F(1)plants displayed restored fertility even withoutRf4. To determine the hidden locus affecting fertility restoration, next-generation sequencing-based QTL-seq was performed with two specific extreme bulks consisting of 30 fertile and 30 sterilerf4rf4individuals from the F(2)population. A major QTL related to fertility restoration, designatedqRf8-1, was detected on the long arm of chromosome 8 in A619. Subsequently,qRf8-1was further validated and narrowed down to a 17.93-Mb genomic interval by insertion and deletion (InDel) and simple sequence repeat (SSR) marker-based traditional QTL mapping, explaining 12.59% (LOD = 25.06) of the phenotypic variation. Thus, using genetic analyses and molecular markers, we revealed another fertility restoration system acting in parallel withRf4in A619 that could rescue the male sterility of CHuangzaosi. This study not only expands the original fertility restoration system but also provides valuable insights into the complex genetic mechanisms underlying the fertility restoration of CMS-C.
- 相关文献
作者其他论文 更多>>
-
Abamectin exposure causes chronic toxicity and trypsin/chymotrypsin damages in Chironomus kiiensis Tokunaga (Diptera: Chironomidae)
作者：Zheng, Xusong;Li, Qiang;Ullah, Farman;Lu, Zhongxian;Mo, Wujia;Guo, Jiawen;Liu, Xiaowei;Xu, Hongxing;Lu, Yanhui
关键词：Abamectin; Toxicity; Serine proteases; Chironomus kiiensis; Rice field
-
Glucosinolate extract from radish (Raphanus sativus L.) seed attenuates high-fat diet-induced obesity: insights into gut microbiota and fecal metabolites
作者：Zhu, Quanfeng;Zhang, Peng;Tang, Leilei;Yu, Jiawen;Jiang, Guojun;Liu, Daqun;Zhang, Chengcheng
关键词：radish seeds; glucosinolate; obesity; gut microbiota; fecal metabolome
-
Evolutionary Origin of the Carotenoid Cleavage Oxygenase Family in Plants and Expression of Pepper Genes in Response to Abiotic Stresses
作者：Yao, Yixiu;Wan, Hongjian;Yao, Yixiu;Cheng, Yuan;Ruan, Meiying;Ye, Qingjing;Wang, Rongqing;Yao, Zhuping;Zhou, Guozhi;Yu, Jiahong;Wan, Hongjian;Jia, Li;Liu, Jia;Yu, Jiahong;Zhang, Peng;Yin, Yuhe;Diao, Weiping;Wan, Hongjian
关键词：pepper; phylogenetic analysis; gene expression; abiotic stress; NCED
-
Recent Progress in the Regeneration and Genetic Transformation System of Cucumber
作者：Tan, Jihong;Lin, Lili;Miao, Li;Wang, Huasen;Tan, Jihong;Zhou, Shengjun;Zhu, Yuqiang;Wang, Xin;Zhang, Peng;Luo, Haiyan
关键词：cucumber; genetic transformation; Agrobacterium tumefaciens; transgenic plants; positive selection system; biotechnology; plant regeneration
-
Comparative analysis of powdery mildew resistant and susceptible cultivated cucumber (Cucumis sativus L.) varieties to reveal the metabolic responses to Sphaerotheca fuliginea infection
作者：Zhang, Peng;Zhu, Yuqiang;Zhou, Shengjun
关键词：Fatty acid; Flavonoid; Hormone; Metabolism; Powdery mildew; Transcription factor
-
Comparative transcriptomic analyses of powdery mildew resistant and susceptible cultivated cucumber (Cucumis sativus L.) varieties to identify the genes involved in the resistance to Sphaerotheca fuliginea infection
作者：Zhang, Peng;Zhu, Yuqiang;Zhou, Shengjun
关键词：Powdery mildew; Cucumber; Differential expressed gene; Hormone; Pathogen resistance
-
Comparative proteomic analysis provides insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.)
作者：Zhang, Peng;Zhu, Yuqiang;Zhou, Shengjun;Luo, Xiujun
关键词：
