文献类型： 外文期刊

作者： Chen, Rui-Yi ¹ ; Hieu, Bui Thi Ngoc ³ ; Audira, Gilbert ³ ; Lou, Bao ⁵ ; Lin, Ming-Der ⁶ ; Hsiao, Chung-Der ³ ;

作者机构： 1.Marine Fisheries Res Inst Zhejiang, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan 316100, Peoples R China

2.Zhejiang Ocean Univ, Marine & Fishery Inst, Zhoushan 316100, Peoples R China

3.Chung Yuan Christian Univ, Dept Biosci Technol, Chungli 32023, Taiwan

4.Chung Yuan Christian Univ, Dept Chem, Chungli 32023, Taiwan

5.Zhejiang Acad Agr Sci, Inst Hydrobiol, Shiqiao Rd 198, Hangzhou 310021, Peoples R China

6.Tzu Chi Univ, Coll Med, Dept Mol Biol & Human Genet, 701 Zhongyang Rd,Sec 3, Hualien 97004, Taiwan

7.Chung Yuan Christian Univ, Ctr Nanotechnol, Chungli 32023, Taiwan

关键词： myoglobin; hemoglobin; fish; meta-transcriptomic

期刊名称：ANIMALS （ 影响因子：2.752； 五年影响因子：2.942 ）

ISSN： 2076-2615

年卷期： 2020 年 10 卷 7 期

页码：

收录情况： SCI

摘要： Simple Summary Oxygen-binding proteins that mediate oxygen-binding for storage and consumption, to reduce energy, are very diverse in fish, depending on their habitats. In the present study, oxygen-binding protein gene expression in the skeletal muscle of 25 diverse fish species was examined by a meta-transcriptomic approach. By using RNAseq data, this is the first study to examine the high level of myoglobin, one of the oxygen-binding proteins, transcripts in pacu and loach fish that might be related to their high tolerance for the oxygen-deficient environment. In addition, this study presents the power of the current method to compare the fish oxygen-binding protein expression and its putative gene expansion event. Oxygen-binding proteins, such as myoglobin, hemoglobin, neuroglobin, and cytoglobin, play a role in oxygen binding and delivery to tissues. In icefish, the loss of myoglobin and hemoglobin genes has been reported to be an adaptive evolution event. This interesting finding prompted us to exam oxygen-binding protein expression in diverse fish species. Taking advantage of substantial RNAseq data deposited in the NCBI (National Center for Biotechnology Information) database, we adopted a meta-transcriptomic approach to explore and compare four oxygen-binding protein gene expression levels in the skeletal muscle of 25 diverse fish species for the first time. RNAseq data were downloaded from the NCBI Sequence Read Archive (SRA) database, and de novo assembly was performed to generate transcript contigs. The genes encoding oxygen-binding proteins were then identified by the BLAST search, and the relative expression level of oxygen-binding protein genes was normalized by the RPKM (Reads per Kilobase Million) method. By performing expression profiling, hierarchy clustering, and principal component analysis, pacu and loach fish were noticed by their high myoglobin expression levels in skeletal muscle tissues among 25 diverse fish species. In conclusion, we demonstrated that meta-transcriptomic analysis of RNAseq data is an informative approach to compare the oxygen-binding protein expression and putative gene expansion event in fish.