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Structural elucidation, anti-inflammatory activity and intestinal barrier protection of longan pulp polysaccharide LPIIa

文献类型： 外文期刊

作者： Bai, Yajuan 1 ; Jia, Xuchao 1 ; Huang, Fei 1 ; Zhang, Ruifen 1 ; Dong, Lihong 1 ; Liu, Lei 1 ; Zhang, Mingwei 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Minist Agr & Rural Affairs, Guangdong Key Lab Agr Prod Proc, Guangzhou 510610, Peoples R China

关键词： Longan pulp polysaccharide; Structural characteristics; NMR; Anti-inflammatory activity; Intestinal barrier protection

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN： 0144-8617

年卷期： 2020 年 246 卷

页码：

收录情况： SCI

摘要： In this study, LPIIa, a purified polysaccharide from longan pulp, was isolated. Its anti-inflammatory activity and intestinal barrier protection were investigated with LPS-treated co-culture model of Caco-2 cells and RAW 264.7 macrophages. The average molecular weight LPIIa was 159.3 kDa. Its detailed structure was shown below. The backbone of LPIIa was composed of (1 -> 3,4)-linked-alpha-Rhap, (1 -> 4)-linked-beta-Galp, (1 -> 6)-linked-beta-Galp, and (1 -> 3,6)-linked-beta-Galp, with branches at the O-4 of Rha and O-3 of Gal, consisting of side chains of alpha-Araf, beta-Galp, and alpha-Glcp. In LPS-induced RAW 264.7 macrophages, LPIIa suppressed the production of inflammatory mediators, including TNF-alpha, IL-6, NO, and PGE2, and inhibited iNOS and COX-2 gene expression. In addition, LPIIa attenuated intestinal tight-junctional channel protein Claudin-2 expression and increased tight-junctional barrier protein ZO-1 expression in Caco-2 cells. Knowing the structural features and activities of longan polysaccharide gives insights into longan polysaccharide application as an anti-inflammatory agent or adjuvant in curing the intestinal inflammation.

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