Sensitive Immunochromatographic Assay Using Highly Luminescent Quantum Dot Nanobeads as Tracer for the Detection of Cyproheptadine Hydrochloride in Animal-Derived Food
文献类型： 外文期刊
作者： Li, Pan 1 ; Yang, Cuifeng 2 ; Liu, Beibei 1 ; Wu, Qin 1 ; Wang, Yulong 1 ; Dong, Sa 1 ; Zhang, Hanxiaoya 1 ; Vasylieva, N 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Minist Agr,Inst Food Safety & Nutr, Key Lab Food Qual & Safety Jiangsu Prov,State Key, Key Lab Control Technol & Stand Agroprod Safety &, Nanjing, Peoples R China
2.Taiyuan Univ, Tourism Dept, Taiyuan, Peoples R China
3.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Jiangxi, Peoples R China
4.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou, Jiangsu, Peoples R China
5.Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA
6.Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA
关键词： immunochromatographic assay; quantum dot nanobeads; cyproheptadine hydrochloride; animal-derived foods; monoclonal antibody
期刊名称：FRONTIERS IN CHEMISTRY （ 影响因子：5.221； 五年影响因子：5.385 ）
ISSN： 2296-2646
年卷期： 2020 年 8 卷
页码：
收录情况： SCI
摘要： Cyproheptadine hydrochloride (CYP), used as human and veterinary drug, has been used illegally as feed additive for food-producing animals, which could remain in food and jeopardize human health. There is a need for on-site detection of CYP residue in animal-derived food. In this study, a hapten was designed, and a specific monoclonal antibody (mAb) was developed to detect CYP with an IC(50)of 1.38 ng/mL and negligible cross-reactivity (CR) for other analogs. Forthermore, a high sensitive immunochromatographic assay (QBs-ICA) was developed using quantum dot nanobeads as reporters. The assay showed the linear detection range (IC20-IC80) of 0.03-0.52 ng/mL, the limit of detection (LOD) and visual detection limit (VDL) reached to 0.01 and 0.625 ng/mL, respectively. Spiked recovery study in pig urine and pork confirmed that the QBs-ICA was applicable for on-site testing. This assay showed better sensitivity and speedy than the reported instrumental analysis and immunoassays.
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