Genetic characterization of a new radish introgression line carrying the restorer gene for Ogura CMS in Brassica napus
文献类型： 外文期刊
作者： Wang, Tonghua 1 ; Guo, Yiming 2 ; Wu, Zengxiang 1 ; Xia, Shengqian 1 ; Hua, Shuijin 4 ; Tu, Jinxing 1 ; Li, Mei 2 ; Chen, 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
2.Hunan Acad Agr Sci, Crop Res Inst, Changsha, Peoples R China
3.Huazhong Agr Univ, Rapeseed Engn Res Ctr, Minist Educ, Wuhan, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2020 年 15 卷 7 期
页码：
收录情况： SCI
摘要： Creating a homologous restorer line for Ogura cytoplasmic male sterility (Ogu-CMS) in Brassica napus is meaningful for the wider application of Ogu-CMS system in rapeseed production. Previously, an independent development of a new Ogu-CMS restorer line (CLR650) was reported locally from crossing between Raphanobrassica (AACCRR, 2n = 56) and B. napus and a new version of Ogu CMS lines CLR6430 derived from CLR650 was characterized in this study. The results showed that the fertility restoration gene in CLR6430 presented a distorted segregation in different segregating populations. However, the majority of somatic cells from roots had a regular chromosome number (2n = 38) and no radish signal covered a whole chromosome was detected using GISH. Thirty-two specific markers derived from the introgressed radish fragments were developed based on the re-sequencing results. Unique radish insertions and differences between CLR6430 and R2000 were also identified through both radish-derived markers and PCR product sequences. Further investigations on the genetic behaviors, interactions between the fertility restoration and other traits and specific molecular markers to the introgression in CLR6430 were also conducted in this study. These results should provide the evidence of nucleotide differences between CLR6430 and R2000, and the specific markers will be helpful for breeding new Ogura restore lines in future.
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