Diversity in metagenomic sequences reveals new pathogenic fungus associated with smut in Job's tears
文献类型： 外文期刊
作者： Li Xiang-dong 1 ; Shi Ming 1 ; Pan Hong 1 ; Lu Xiu-juan 1 ; Wei Xin-yuan 1 ; Lu Ping 3 ; Lian Qi-xian 4 ; Fu Yu-hua 5 ;
作者机构： 1.Southwest Guizhou Inst Karst Reg Dev, Xingyi 562400, Peoples R China
2.Adlay Engn Tech Res Ctr Guizhou, Xingyi 562400, Peoples R China
3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
4.Xingyi Normal Univ Nationalities, Xingyi 562400, Peoples R China
5.Guizhou Acad Agr Sci, Guizhou Inst Subtrop Crops, Xingyi 562400, Peoples R China
关键词： metagenomic; ITS; smut; Job's tears
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2020 年 19 卷 9 期
页码：
收录情况： SCI
摘要： Smut is a serious disease in Job's tears, also known as adlay, and contributes to the reduction of crop yield in agricultural fields. In this study, the key pathogenic fungi in adlay smut disease were first identified by internal transcribed spacer region (ITS) high-throughput sequencing and then used to elucidate the composition and diversity of the fungal community in adlay smut. Results indicate that an abundance of operational taxonomic units (OTUs) were detected in the infected involucres of flowering plants and the OTUs were classified to nine phyla, 20 classes, 45 orders, 90 families and 119 genera. A total of 4 986 OTUs clustered together, sharing six core OTUs in all samples, while 145 OTUs were shared among four geographical groups. The Shannon and Simpson indices ranged from 0.137-1.629 and 0.357-0.970, respectively. Community diversity ranked as Anshun (AS) Qinglong (QL)>Xingren (XR)>Xingyi (XY) among the four geographical groups by Shannon and Simpson indices, exhibiting complex community diversities among accessions and geographical groups. The richness and diversity data imply a weak relationship between the accession community richness and geographical origins of samples. Two closely related fungal genera, Sporisorium and Ustilago, were implicated as causes of smut disease. The genus Sporisorium appears to be more commonly found among accessions and thus is more likely to be the fungal pathogen causing smut in adlay. This work can facilitate strategies to control and prevent smut infection to improve adlay yield.
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