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A cost-effective barcode system for maize genetic discrimination based on bi-allelic InDel markers

文献类型： 外文期刊

作者： Liang, Shuaiqiang 1 ; Lin, Feng 1 ; Qian, Yiliang 2 ; Zhang, Tifu 1 ; Wu, Yibo 1 ; Qi, Yaocheng 2 ; Ren, Sihai 2 ; Ruan, 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Crop Germplasm & Biotechnol, Prov Key Lab Agrobiol, Nanjing, Peoples R China

2.Anhui Acad Agr Sci, Hefei, Peoples R China

关键词： Maize; Barcode system; InDel marker; Genetic discrimination

期刊名称：PLANT METHODS （ 影响因子：4.993； 五年影响因子：5.312 ）

ISSN：

年卷期： 2020 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Background Maize is one of the most important cereal crop all over the world with a complex genome of about 2.3 gigabase, and exhibits tremendous phenotypic and molecular diversity among different germplasms. Along with the phenotype identification, molecular markers have been accepted extensively as an alternative tool to discriminate different genotypes. Results By using previous re-sequencing data of 205 lines, bi-allelic insertions and deletions (InDels) all over maize genome were screened, and a barcode system was constructed consisting of 37 bi-allelic insertion-deletion markers with high polymorphism information content (PIC) values, large discriminative size among varieties. The barcode system was measured and determined, different maize hybrids and inbreds were clearly discriminated efficiently with these markers, and hybrids responding parents were accurately determined. Compared with microarray data of more than 200 maize lines, the barcode system can discriminate maize varieties with 1.57% of different loci as a threshold. The barcode system can be used in standardized easy and quick operation with very low cost and minimum equipment requirements. Conclusion A barcode system was constructed for genetic discrimination of maize lines, including 37 InDel markers with high PIC values and user-friendly. The barcode system was measured and determined for efficient identification of maize lines.

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