QTL mapping and GWAS for field kernel water content and kernel dehydration rate before physiological maturity in maize
文献类型： 外文期刊
作者： Li, Shufang 1 ; Zhang, Chunxiao 1 ; Lu, Ming 2 ; Yang, Deguang 3 ; Qian, Yiliang 4 ; Yue, Yaohai 2 ; Zhang, Zhijun 2 ; Ji 1 ;
作者机构： 1.Jilin Acad Agr Sci, Crop Germplasm Resources Inst, Kemaoxi St 303, Gongzhuling 136100, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Maize Res Inst, Gongzhuling 136100, Peoples R China
3.Northeast Agr Univ, Coll Agron, Harbin 150030, Peoples R China
4.Anhui Acad Agr Sci, Maize Res Ctr, Hefei 230001, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2020 年 10 卷 1 期
页码：
收录情况： SCI
摘要： Kernel water content (KWC) and kernel dehydration rate (KDR) are two main factors affecting maize seed quality and have a decisive influence on the mechanical harvest. It is of great importance to map and mine candidate genes related to KWCs and KDRs before physiological maturity in maize. 120 double-haploid (DH) lines constructed from Si287 with low KWC and JiA512 with high KWC were used as the mapping population. KWCs were measured every 5 days from 10 to 40 days after pollination, and KDRs were calculated. A total of 1702 SNP markers were used to construct a linkage map, with a total length of 1,309.02 cM and an average map distance of 0.77 cM. 10 quantitative trait loci (QTLs) and 27 quantitative trait nucleotides (QTNs) were detected by genome-wide composite interval mapping (GCIM) and multi-locus random-SNP-effect mixed linear model (mrMLM), respectively. One and two QTL hotspot regions were found on Chromosome 3 and 7, respectively. Analysis of the Gene Ontology showed that 2 GO terms of biological processes (BP) were significantly enriched (P <= 0.05) and 6 candidate genes were obtained. This study provides theoretical support for marker-assisted breeding of mechanical harvest variety in maize.
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