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Expression Analysis, Functional Marker Development and Verification of AgFNSI in Celery

文献类型： 外文期刊

作者： Yan, Jun 1 ; He, Lizhong 1 ; Xu, Shuang 1 ; Wan, Yanhui 1 ; Wang, Hong 1 ; Wang, Ying 1 ; Yu, Li 1 ; Zhu, Weimin 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Hort Res Inst, Shanghai 201403, Peoples R China; Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2020 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Apigenin is one of the primary flavonoids in celery, which has a high medicinal value. Flavone synthase I (FNSI) is the last step enzyme in apigenin biosynthesis. In this study, the 1492 bp promoter sequence before AgFNSI initiation codon (ATG) of celery was obtained, which included methyl jasmonate (MeJA) responsive elements, light responsive elements, anaerobic induction elements and five MYB binding sites. AgFNSI was sensitive to temperature, UV-B, water deficit and MeJA. Comparative analysis of AgFNSI genome and promoter sequences among celery accessions with different apigenin content showed that there were four allelic variations in AgFNSI, and four accessions with high apigenin content belonged to AgFNSIa, and five accessions with low apigenin content belonged to AgFNSIc. Three pairs of dominant complementary markers were designed based on the single-nucleotile polymorphisms (SNPs) of the AgFNSIa and AgFNSIc genomes and promoter sequences. Three pairs of functional markers were validated by 112 celery accessions. The results showed that AFPA1/AFPB1 detected significant differences in apigenin content between different genotypes. Therefore, marker AFPA1/ AFPB1 is associated with apigenin content in celery and could be used for the genetic improvement of apigenin content in celery.

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