文献类型： 外文期刊

作者： Zhao, Ming-Min ¹ ; Lyu, Ning ¹ ; Wang, Dong ¹ ; Wu, Xiao-Gang ² ; Zhao, Yuan-Zheng ⁴ ; Zhang, Li-Qun ² ; Zhou, Hong-Yo ¹ ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Hort & Plant Protect, Hohhot 010019, Inner Mongolia, Peoples R China

2.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

3.Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R China

4.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Inner Mongolia, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2020 年 10 卷 1 期

页码：

收录情况： SCI

摘要： The antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG), produced by the Gram-negative rod-shaped bacterium Pseudomonas fluorescens 2P24, is active against various soil-borne bacterial and fungal pathogens that cause plant diseases. Biosynthesis of 2,4-DAPG is controlled by regulating expression of the phlACBD operon at the post-transcriptional level. The phlG gene is located between the phlF and phlH genes, upstream of the phlACBD biosynthetic operon. Herein, we cloned the phlG gene, generated a phlG deletion mutant, and investigated its regulatory role in 2,4-DAPG biosynthesis. The results showed that deletion of phlG had no effect on the biosynthesis of 2,4-DAPG, but it affected conversion of 2,4-DAPG to its precursor monoacetylphloroglucinol (MAPG). The global regulatory factor encoded by gacS positively regulated expression of phlG, while rsmE negatively regulated its expression. Deleting phlG did not alter the ability of the bacterium to colonise plants or promote plant growth. These results suggest that phlG collaborates with other factors to regulate production of the antibiotic 2,4-DAPG in P. fluorescens 2P24.