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Expression and characterization of a novel glycoside hydrolase family 46 chitosanase identified from marine mud metagenome

文献类型： 外文期刊

作者： Sun, Huihui 1 ; Yang, Guosong 2 ; Cao, Rong 1 ; Mao, Xiangzhao 2 ; Liu, Qi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Dept Food Engn & Nutr, Qingdao 266071, Peoples R China

2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China

关键词： Chitosanase; GH46 family; Metagenome

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 159 卷

页码：

收录情况： SCI

摘要： A novel chitosanase gene, csn4, was identified through function-based screening of a marine mud metagenomic library. The encoded protein, named CSN4, which belonged to glycoside hydrolase family 46, showed its maximum identity (79%) with Methylobucter tundripuluduin peptidoglycan-binding protein. CSN4 was expressed in Eschericliia coli and purified. It displayed maximal activity at 30 degrees C and pH 7. A weakly-alkaline solution strongly inhibited the activity. The enzymatic activity was enhanced by addition of Mn2+ or Co2+. CSN4 exhibited strict substrate specificity for chitosan, and the activity was enhanced by increasing the degree of deacetylation. Thin-layer chromatography and electrospray ionization-mass spectrometry showed that CSN4 displayed an endo-type cleavage pattern, hydrolyzing chitosan mainly into (GlcN)(2), (GlcN)(3) and (GlcN)(4). The novel characteristics of the chitosanase CSN4 make it a potential candidate to produce chitooligosaccharides from chitosan in industry, (C) 2020 Published by Elsevier B.V.

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