Transcriptome and Flavonoids Metabolomic Analysis Identifies Regulatory Networks and Hub Genes in Black and White Fruits ofLycium ruthenicumMurray
文献类型： 外文期刊
作者： Li, Tingting 1 ; Fan, Yunfang 2 ; Qin, Huan 1 ; Dai, Guoli 2 ; Li, Guoxiu 1 ; Li, Yanlong 2 ; Wang, Jingjin 1 ; Yin, Yue; 1 ;
作者机构： 1.Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu, Peoples R China
2.Ningxia Acad Agr & Forestry Sci, Inst Wolfberry Engn Technol, Yinchuan, Ningxia, Peoples R China
3.Natl Wolfberry Engn Technol Res Ctr, Yinchuan, Ningxia, Peoples R China
关键词： anthocyanin; fruits; flavonoid; Lycium ruthenicum; metabolic profiling; transcriptome
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）
ISSN： 1664-462X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： Lycium ruthenicumMurry. is a highly nutritional cash crop due to its fruit abundant anthocyanins. To understand the complex metabolic networks underlying the color formation in black and white fruits ofL. ruthenicum, we conducted transcriptome and flavonoid metabolic profiling to identify the candidate genes possibly involved in flavonoid biosynthesis. As a result, 147 flavonoids were identified and there was almost no anthocyanin in white fruits, while luteolin, kaempferol, and quercetin derivatives showed markedly higher abundance. Furthermore, applying weighted gene co-expression network analyses, 3 MYB, 2 bHLH, 1WRKY and 1 NAC transcription factor, associated with anthocyanin biosynthesis were identified. A bHLH transcription factor,LrAN1bshowed the greatest correlations with anthocyanin accumulation with no expression in white fruits. In addition, gene function analysis and qRT-PCR experiments identified a new activated anthocyanin MYB transcription factor designed asLrAN2-like. Yeast two-hybrid and transient tobacco overexpression experiments showed thatLrAN1bcould interact withLrAN2-likeandLrAN11to form MBW complex to activate the anthocyanin pathway. The yeast one-hybrid experiment indicated thatLrAN2-likebonded anthocyanin structural geneLrDFRandLrANSpromoters. Heterologous expression ofLrAN1bin tobacco can significantly increase the anthocyanin content of tobacco florals and capsules, and activate anthocyanin synthesis related genes. Taken together, an anthocyanin regulatory network model inL. ruthenicumfruit was proposed firstly and we speculate that the white fruit phenotype was due to abnormal expression ofLrAN1b. The findings provide new insight into the underlying mechanism of flavonoids, laying the foundation for future functional and molecular biological research inL. ruthenicum.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of LbPYL10 in Heat Tolerance in Goji (Lycium barbarum)
作者：Li, Zeyu;Liu, Jiyao;Chen, Yan;He, Wei;Liang, Aihua;Mu, Zixin;Liang, Aihua;Mu, Zixin;Liang, Aihua;Mu, Zixin;Qin, Xiaoya;Qin, Ken
关键词：Lycium barbarum; ABA signaling; PYL/RCAR ABA receptors; genome-wide; heat stress
-
In vivo absorption and fecal excretion of polysaccharides from the fruits of Lycium barbarum L. in rats through fluorescence labeling
作者：Zhou, Wangting;Kan, Xuhui;Dong, Wei;Sun, Yi;Zeng, Xiaoxiong;Yan, Yamei;Mi, Jia;Lu, Lu;Cao, Youlong;Wang, Wei
关键词：Lycium barbarum polysaccharides; Fluorescence labeling; Absorption
-
Wolfberry genome database: integrated genomic datasets for studying molecular biology
作者：Cao, You-Long;Li, Yan-Long;Fan, Yun-Fang;Luo, Qing;Zhao, Jian-Hua;Yin, Yue;An, Wei;Shi, Zhi-Gang;Cao, You-Long;Li, Yan-Long;Fan, Yun-Fang;Luo, Qing;Zhao, Jian-Hua;Yin, Yue;An, Wei;Shi, Zhi-Gang;Chen, You-Yi;Li, Chung-, I;Lin, Shao-Ting;Tsai, Wen-Chieh;Lin, Shao-Ting;Tsai, Wen-Chieh;Lee, Bing-Ru;Hsieh, Chun-Lin;Wu, Wei-Sheng;Hsiao, Yu-Yun;Tsai, Wen-Chieh;Chow, Chi-Nga;Chang, Wen-Chi;Tsai, Wen-Chieh;Huang, Chun-Lin;Chang, Wei-Hung;Chang, Wei-Hung;Liu, Zhong-Jian;Liu, Zhong-Jian;Liu, Zhong-Jian;Liu, Zhong-Jian;Tsai, Wen-Chieh;Tsai, Wen-Chieh
关键词：wolfberry; Lycium barbarum; wolfberry genome database (WGDB); user-friendly; genomics; transcriptomics
-
Petunidin-3-O-[rhamnopyranosyl-(trans-p-coumaroyl)]-5-O-(β-D-glucopyranoside), the main anthocyanin from the fruits of Lycium ruthenicum murray, enhances the lifespan of Caenorhabditis elegans by activating DAF-16 and improving the gut microbiota
作者：Zhang, Kangzhi;Zhu, Jiahao;Liu, Peiyun;Guo, Renpeng;Zeng, Xiaoxiong;Yan, Yamei;Mi, Jia;Lu, Lu;Cao, Youlong;Yan, Yamei;Mi, Jia;Lu, Lu;Cao, Youlong
关键词：Lycium ruthenicum murray; Caenorhabditis elegans; Lifespan; Antioxidant; Resistance; DAF-16; Intestinal microbiota
-
Impact of Different Rates of Nitrogen Supplementation on Soil Physicochemical Properties and Microbial Diversity in Goji Berry
作者：Liang, Xiaojie;Cui, Yanhong;Su, Shuchai;Liang, Xiaojie;An, Wei;Li, Yuekun;Wang, Yajun;Qin, Xiaoya
关键词：Goji berry production; Ningxia; China; differential nitrogen supplementation rates; 16S RNA gene and IT1 & IT2 region sequencing; soil physicochemical properties
-
Localization of S-Locus-Related Self-Incompatibility in Lycium barbarum Based on BSA Analysis
作者：Wang, Cuiping;Wu, Jiali;Shang, Xiaohui;Ma, Haijun;Zhang, Xin;Xu, Wendi;Wang, Cuiping;Gao, Yan;Dai, Guoli;Qin, Ken;Ma, Haijun
关键词：Lycium barbarum; self-incompatibility; S locus; bulked segregant analysis
-
Mapping quantitative trait loci associated with self-(in)compatibility in goji berries (Lycium barbarum)
作者：Wang, Cuiping;Shang, Xiaohui;Wu, Jiali;Ma, Haijun;Wei, Zhaojun;Wang, Cuiping;Qin, Ken;Gao, Yan;Dai, Guoli;Ma, Haijun
关键词：Lycium barbarum; Genetic map; Self-incompatibility; Quantitative trait loci; S-factor
