文献类型： 外文期刊

作者： Ren, Zhuling ¹ ; Wang, Shifeng ¹ ; Cai, Yan ¹ ; Wu, Yue ¹ ; Tian, Liangjin ¹ ; Liao, Jingqiu ¹ ; Wang, Shaoqun ¹ ; Jiang, ¹ ;

作者机构： 1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China

2.Key Lab Trop Hydrobiol & Biotechnol Hainan Prov, Haikou 570228, Hainan, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China

关键词： Aeromonas schubertii; Nile tilapia; Histopathology; Immune related genes

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2020 年 529 卷

页码：

收录情况： SCI

摘要： Aeromonas schubertii is a conditional pathogen widely distributed in aquatic environments, yet it was only recently reported as a causative pathogen in fish. This study investigated antioxidant capacity, non-specific immunity, histopathology and immune-related gene expression profiles in Nile tilapia Oreochromis niloticus injected with A. schubertii. The results show that A. schubertii infection stimulated the activation of antioxidant activity and non-specific immunity in Nile tilapia. Histopathological analysis revealed that inflammation existed in the liver, spleen, head kidney, brain and intestine of the infected fish. Quantitative real-time PCR was performed to measure mRNA expression levels of six immune-related genes (interleukin-1 beta, tumor necrosis factor-alpha, cyclooxygenase-2, transforming growth factor beta, interleukin-10 and heat shock protein 70) in the intestine and head kidney at different timepoints after experimental infection. The results demonstrated clear transcriptional activation of these immune-related genes in the intestine and head-kidney tissues. To our knowledge, this is the first study to investigate the host response to A. schubertii infection in a fish. These results will contribute to further understanding of the pathogenesis and host defense system in fishes following infection with A. schubertii.