Transcriptome-wide analysis of the AP2/ERF transcription factor gene family involved in the regulation of gypenoside biosynthesis in Gynostemma pentaphyllum
文献类型： 外文期刊
作者： Xu, Shiqiang 2 ; Yao, Shaochang 1 ; Huang, Rongshao 1 ; Tan, Yong 1 ; Huang, Ding 1 ;
作者机构： 1.Guangxi Univ Chinese Med, Coll Pharm, Nanning 530200, Guangxi, Peoples R China
2.Guangdong Acad Agr Sci, Crops Res Inst, Guangdong Prov Key Lab Crops Genet & Improvement, Guangzhou 510640, Peoples R China
关键词： AP2/ERF; Transcription factor; Gynostemma pentaphyllum; Gypenosides; Tissue-specific; Expression pattern
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）
ISSN： 0981-9428
年卷期： 2020 年 154 卷
页码：
收录情况： SCI
摘要： Gynostemma pentaphyllum is a traditional Chinese medicinal herb, serving as natural source of gypenosides (triterpene saponins). The APETALA2/ethylene response factor (AP2/ERF) transcription factors, playing essential regulation roles in plant biotic and abiotic stress responses and secondary metabolism biosynthesis. However, the regulation roles of AP2/ERF transcription factors in gypenosides biosynthesis in G. pentaphyllum remains little understood. In the present study, 125 AP2/ERF genes were identified from G. pentaphyllum transcriptome datasets. Phylogenetic, conserved motifs and expression pattern were employed to comprehensively analyze the 125 GpAP2/ERF genes. Based on the sequence similarity and phylogeny tree, the 125 GpAP2/ERF genes can be classified into 10 groups. Moreover, the distribution of conserved motifs among GpAP2/ERF proteins in phylogenetic trees was consistent with previous studies, thus supporting the classification. Expression profiling indicated that the 125 GpAP2/ERF genes exhibited distinct tissue-specific expression patterns. As confirmed by qRT-PCR, the four candidate GpAP2/ERF genes and gypenoside biosynthetic genes were highly expressed in leaves and/or flowers, and show similar expression patterns in response to MeJA. Base on the expression patterns and phylogenetic relationships, two GpAP2/ERF genes were considered as potential regulatory genes for gypenoside biosynthesis. Our study enhances understanding roles of GpAP2/ERF genes in regulation of gypenosides biosynthesis.
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