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M-like protein SrM is not crucial to the virulence of a novel isolate of Streptococcus equi subsp. ruminatorum from Macaca mulatta

文献类型： 外文期刊

作者： Guo, Genglin 1 ; Wei, Dan 1 ; Zhang, Yuhang 1 ; Wang, Kaicheng 4 ; Liu, Rongchang 5 ; Wu, Zhiqiang 6 ; Kong, Lifang 6 ; C 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

2.Minist Agr, Key Lab Anim Bacteriol, Nanjing 210095, Peoples R China

3.OIE Reference Lab Swine Streptococcosis, Nanjing 210095, Peoples R China

4.China Anim Hlth & Epidemiol Ctr, Qingdao, Shandong, Peoples R China

5.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China

6.City Pk Management Off Nanping City, Nanping 353000, Fujian, Peoples R China

关键词： Streptococcus equi subsp. ruminatorum; M-like protein; Virulence; Zoonosis

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN： 0034-5288

年卷期： 2020 年 132 卷

页码：

收录情况： SCI

摘要： In this study, a Streptococcus strainnamed FJ1804, was isolated from a blood sample collected from a dead Macaca mulatta in China and, was subsequently classified as Streptococcus equi subsp. ruminatorum (S.e. ruminatorum) through 16S rRNA gene sequence analysis. After whole genome sequencing and analysis, an M-like protein encoding gene that encodes an SrM protein that is homologous to the crucial S.e. zooepidemicus crucial virulence factor SzP, was identified in the genome of FJ1804. To determinethe function of SrM in this bacterium, a strain deleted of srm as well as a complement strain were constructed. The results of in vitro cell adherence, invasion and phagocytosis assays and in vivo animal challenge and histopathology showed that the anti-phagocytosis was decreased and the adherence rate was increased in the srm deletion strain, whereas the invasion rate, pathological features and LD50 values inboth zebrafish and BALB/c mice model showed no difference compared to that observed for the WT strain. To the best of our knowledge, this is first of an infection caused by S.e. ruminatorum, which is a newly identified zoonotic pathogen, in Macaca mulatta, and our data suggest that, compared with other S.e. zooepidemicus strains, the SzP homologous protein is not crucial to the virulence of this bacterium.

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