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Identification ofAvramr1fromPhytophthora infestansusing long read and cDNA pathogen-enrichment sequencing (PenSeq)

文献类型： 外文期刊

作者： Lin, Xiao 1 ; Song, Tianqiao 1 ; Fairhead, Sebastian 1 ; Witek, Kamil 1 ; Jouet, Agathe 1 ; Jupe, Florian 1 ; Witek, A 1 ;

作者机构： 1.Univ East Anglia, Sainsbury Lab, Norwich, Norfolk, England

2.Wageningen Univ & Res, Plant Breeding, Wageningen, Netherlands

3.Univ Dundee, Sch Life Sci, Div Plant Sci, Dundee, Scotland

4.James Hutton Inst, Cell & Mol Sci, Dundee, Scotland

5.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Peoples R China

6.Bayer Crop Sci, Chesterfield, MO USA

7.ARS, USDA, Madison, WI USA

关键词： Avramr1; late blight; oomycete; PenSeq; Phytophthora infestans; Rpi-amr1; RXLR effector

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期：

页码：

收录情况： SCI

摘要： Potato late blight, caused by the oomycete pathogenPhytophthora infestans, significantly hampers potato production. Recently, a newResistance to Phytophthora infestans(Rpi) gene,Rpi-amr1, was cloned from a wildSolanumspecies,Solanum americanum. Identification of the corresponding recognized effector (AvirulenceorAvr) genes fromP. infestansis key to elucidating their naturally occurring sequence variation, which in turn informs the potential durability of the cognate late blight resistance. To identify theP. infestanseffector recognized byRpi-amr1, we screened available RXLR effector libraries and used long read and cDNA pathogen-enrichment sequencing (PenSeq) on fourP. infestansisolates to explore the untested effectors. Using single-molecule real-time sequencing (SMRT) and cDNA PenSeq, we identified 47 highly expressed effectors fromP. infestans, including PITG_07569, which triggers a highly specific cell death response when transiently coexpressed withRpi-amr1inNicotiana benthamiana, suggesting thatPITG_07569isAvramr1. Here we demonstrate that long read and cDNA PenSeq enables the identification of full-length RXLR effector families and their expression profile. This study has revealed key insights into the evolution and polymorphism of a complex RXLR effector family that is associated with the recognition byRpi-amr1.

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