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Characterization and Fine Mapping of a Yellow-Virescent Gene Regulating Chlorophyll Biosynthesis and Early Stage Chloroplast Development inBrassica napus

文献类型： 外文期刊

作者： Zhao, Chuanji 1 ; Liu, Lijiang 1 ; Safdar, Luqman Bin 1 ; Xie, Meili 1 ; Cheng, Xiaohui 1 ; Liu, Yueying 1 ; Xiang, Ya 1 ;

作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Oil Crops, Oil Crops Res Inst, Wuhan 430062, Peoples R China

2.Huazhong Agr Univ, Natl Ctr Rapeseed Improvement, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

3.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang 550008, Peoples R China

关键词： Brassica napus; leaf color; chlorophyll biosynthesis; chloroplast development; BSA-seq; gene cloning; RNA-seq

期刊名称：G3-GENES GENOMES GENETICS （ 影响因子：3.154； 五年影响因子：3.369 ）

ISSN： 2160-1836

年卷期： 2020 年 10 卷 9 期

页码：

收录情况： SCI

摘要： Chlorophyll biosynthesis and chloroplast development are crucial to photosynthesis and plant growth, but their regulatory mechanism remains elusive in many crop species. We isolated aBrassica napusyellow-virescent leaf (yvl) mutant, which exhibited yellow-younger-leaf and virescent-older-leaf with decreased chlorophyll accumulation and delayed chloroplast development. We mappedyvllocus to a 70-kb interval between molecular markers yvl-O10 and InDel-O6 on chromosome A03 in BC(2)F(2)population using whole genome re-sequencing and bulked segregant analysis. The mutant had a 'C' to 'T' substitution in the coding sequence ofBnaA03.CHLH, which encodes putative H subunit of Mg-protoporphyrin IX chelatase (CHLH). The mutation resulted in an imperfect protein structure and reduced activity of CHLH. It also hampered the plastid encoded RNA polymerase which transcribes regulatory genes of photosystem II and I. Consequently, the chlorophyll a/b and carotenoid contents were reduced and the chloroplast ultrastructure was degraded inyvlmutant. These results explain that a single nucleotide mutation inBnaA03.CHLHimpairs PEP activity to disrupt chloroplast development and chlorophyll biosynthesis inB. napus.

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