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Poly(3-hydroxybutyrate-3-hydroxyvalerate) production from pretreated waste lignocellulosic hydrolysates and acetate co-substrate

文献类型： 外文期刊

作者： Yin, Fen 1 ; Li, Dongna 1 ; Ma, Xiaojun 1 ; Li, Jianing 2 ; Qiu, Yujuan 1 ;

作者机构： 1.Tianjin Univ Sci & Technol, Coll Light Ind Sci & Engn, Tianjin 300222, Peoples R China

2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Biol & Genet Resource Utilizat Rubber Tre, Minist Agr,State Key Lab Breeding Base Cultivat &, Danzhou 571737, Peoples R China

关键词： Lignocellulose; Co-substrate; Activated sludge; PHBV

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

ISSN： 0960-8524

年卷期： 2020 年 316 卷

页码：

收录情况： SCI

摘要： The purpose of this study was to explore the potential of producing Poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by mixed microbial culture (MMC) with lignocellulosic hydrolysates and acetate co-substrate as feedstock. The addition of co-substrate acetate led to the introduction of HV monomer into the polyhydroxyalkanoate (PHA), and the initial mixed sludge suspension (MLSS) increased with the increase of acetate. Almost 1.91-fold increase in the yield of PHA was achieved with limited nitrogen medium (the carbon to nitrogen ratio (C/N) was 33) compared to the normal nitrogen medium (C/N = 20). Limiting nitrogen source and micro alkaline culture environment was more conducive to the accumulation of PHBV. PHA production achieved to the highest value of about 2308.45 mg/L under the condition of optimized technology. Acidovorax was the dominant genus of all bioreactors using co-substrate. Further, utilizing lignocellulosic hydrolysate and acetate co-substrate as feedstock in mixed microbial culture was a promising approach in a low-cost large-scale PHA production.

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