Characterization of Radiation-Resistant Yeast Isolated from Radiation-Polluted Areas and Its Potential Application in Bioremediation
文献类型： 外文期刊
作者： Liu, T. 1 ; Liu, X. 1 ; Zhu, J. 3 ; Tang, Q. 3 ; Wang, W. 3 ; Zhu, L. 4 ; Zhang, Z. 3 ; Zhang, Z. 3 ; Jiang, L. 1 ; Huang, H. 2 ;
作者机构： 1.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 210009, Peoples R China
2.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Peoples R China
3.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi, Xinjiang Uigur, Peoples R China
4.Nanjing Tech Univ, Coll Pharmaceut Sci, Nanjing 210009, Peoples R China
5.Nanjing Beishengrong Energy Technol Co Ltd, Nanjing 210009, Peoples R China
关键词： Cryptococcus; radiation resistance; heavy metal resistance; bioremediation
期刊名称：APPLIED BIOCHEMISTRY AND MICROBIOLOGY （ 影响因子：0.886； 五年影响因子：0.956 ）
ISSN： 0003-6838
年卷期： 2020 年 56 卷 5 期
页码：
收录情况： SCI
摘要： Twenty-four yeast isolates were obtained from radiation-polluted areas soils samples exposed to(60)Co-gamma radiation at a dose of 10 kGy and identified as members of the genusCryptococcusbased on morphological and LSU rDNA D1/D2 domain sequence analysis. Further phylogenetic analysis showed that the isolates were clustered into 4 branches. The investigation of these yeast isolates revealed D(10)values for gamma radiation of 5 (M2), 5 (M38), 6 (M22) and 7 kGy (M5) and a 4 x 10(-3)% survival rate at a UV dosage of 200 J/m(2). Furthermore, the tolerance of M5 towards Zn(2+)was as high as 1.700 mg/L. The isolate M5 was selected for further study, including the distribution of heavy metals within the cells by subcellular fractionation, expression of relevant genes involved in heavy metal resistance by qRT-PCR, and the removal rate by flame atomic absorption spectrometry (FAAS). The results revealed that almost 72.9% of the Zn(2+)was absorbed by the cells and deposited mainly in the membrane fraction. Compared to normal conditions, all of the genes in M5 strain related to transport and regulation showed greater than 10.4-fold increases of expression levels upon exposure to heavy metal stress. In the presence of culture medium, a total of 86.5% of the initial concentration of Zn(2+)was removed by the M5 strain exhibiting high removal efficiency and providing a potential of radiation-resistant platform strain for the bioremediation of heavy metals in polluted environments in the near future.
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