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Full-length genome sequence of segmented RNA virus from ticks was obtained using small RNA sequencing data

文献类型： 外文期刊

作者： Xu, Xiaofeng 1 ; Bei, Jinlong 2 ; Xuan, Yibo 1 ; Chen, Jiayuan 4 ; Chen, Defu 4 ; Barker, Stephen C. 5 ; Kelava, Samue 1 ;

作者机构： 1.Hebei Normal Univ, Coll Life Sci, Hebei Key Lab Anim Physiol Biochem & Mol Biol, Shijiazhuang 050024, Hebei, Peoples R China

2.Guangdong Acad Agr Sci, Guangdong Prov Key Lab Crop Germplasm Resources P, Lab Crop Germplasm Resources Preservat & Utilizat, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China

3.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Guangdong, Peoples R China

4.Nankai Univ, Coll Life Sci, Tianjin 300071, Tianjin, Peoples R China

5.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

关键词： MGTV; JMTV; Full-length genome; 5 ' sRNA; 3 ' sRNA

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background: In 2014, a novel tick-borne virus of theFlaviviridaefamily was first reported in the Mogiana region of Brazil and named the Mogiana tick virus (MGTV). Thereafter, the Jingmen tick virus (JMTV), Kindia tick virus (KITV), and Guangxi tick virus (GXTV)-evolutionarily related to MGTV-were reported. Results: In the present study, we used small RNA sequencing (sRNA-seq) to detect viruses in ticks and discovered a new MGTV strain inAmblyomma testudinariumticks collected in China's Yunnan Province in 2016. We obtained the full-length genome sequence of this MGTV strain Yunnan2016 (GenBank: MT080097, MT080098, MT080099 and MT080100) and recommended it for its inclusion in the NCBI RefSeq database for future studies on MGTV, JMTV, KITV and GXTV. Phylogenetic analysis showed that MGTV, JMTV, KITV and GXTV are monophyletic and belong to a MGTV group. Furthermore, this MGTV group of viruses may be phylogenetically related to geographical regions that were formerly part of the supercontinents Gondwana and Laurasia. Conclusions: To the best of our knowledge, this is the first study in which 5 ' and 3 ' sRNAs were used to generate full-length genome sequences of, but not limited to, RNA viruses. We also demonstrated the feasibility of using the sRNA-seq based method for the detection of viruses in pooled two and even possible one small ticks. MGTV may preserve the characteristic of ancient RNA viruses, which can be used to study the origin and evolution of RNA viruses. In addition, MGTV can be used as novel species for studies in phylogeography.

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