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Transcriptome Analysis of Different Tissues Reveals Key Genes Associated With Galanthamine Biosynthesis inLycoris longituba

文献类型： 外文期刊

作者： Li, Qingzhu 1 ; Xu, Junxu 1 ; Yang, Liuyan 1 ; Zhou, Xiaohui 1 ; Cai, Youming 1 ; Zhang, Yongchun 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Protected Hort Technol, Forestry & Pomol Res Inst, Shanghai, Peoples R China

关键词： Lycoris longituba; galanthamine biosynthesis; transcriptomics; O-methyltransferase; functional characterization

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Lycoris longitubais a traditional medicinal plant containing the bioactive compound galanthamine (Gal), a type of Amaryllidaceae alkaloid and can be used to treat Alzheimer's disease. However, research on its genome or transcriptome and associated genes in the biosynthetic pathway is incomplete. In this study, we estimated the nuclear genome size of this species to be 29.33 Gb by flow cytometry. Then, RNA sequencing of the leaves, roots, and bulbs ofL. longitubawas carried out. Afterde novoassembly, 474,589 all-transcripts and 333,440 all-unigenes were finally generated. In addition, the differentially expressed genes (DEGs) were identified, and genes involved in the galanthamine metabolic pathway encoding tyrosine decarboxylase (TYDC), phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), p-coumarate 3-hydroxylase (C3H), norbelladine synthase (NBS), norbelladine 4'-O-methyltransferase (OMT), and noroxomaritidine synthase (CYP96T1) were detected and validated by real-time quantitative PCR analysis. One candidate gene,Lycoris longitubaO-Methyltransferase (LlOMT), was identified in the proposed galanthamine biosynthetic pathway. Sequence analysis showed that LlOMT is a class I OMT. LlOMT is localized in the cytoplasm, and biochemical analysis indicated that the recombinant LlOMT catalyzes norbelladine to generate 4 '-O-methylnorbelladine. The protoplast transformation result showed that the overexpression of LlOMT could increase the Gal content. Our results indicate that LlOMT may play a role in galanthamine biosynthesis inL. longituba. This work provides a useful resource for the metabolic engineering of Amaryllidaceae alkaloids.

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