Effect of different floatation solutions on E. tenella oocyst purification and optimization of centrifugation conditions for improved recovery of oocysts and sporocysts
文献类型： 外文期刊
作者： Qi, Nanshan 1 ; Liao, Shenquan 1 ; Abuzeid, Asmaa Mi 3 ; Li, Juan 1 ; Wu, Caiyan 1 ; Lv, Minna 1 ; Lin, Xuhui 1 ; Hu, Jun 1 ;
作者机构： 1.Minist Agr, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Key Lab Livestock Dis Prevent Guangdong Prov, Beijing, Peoples R China
2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Guangdong, Peoples R China
3.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China
关键词： Eimeria tenella; Sodium hypochlorite; Purification; Oocysts; Sporocysts
期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）
ISSN： 0014-4894
年卷期： 2020 年 217 卷
页码：
收录情况： SCI
摘要： Saturated salt floatation method is widely used for coccidian oocyst purification. However, the repeated procedures and inefficient oocysts recovery rate are a continuous challenge. This study aimed to investigate the best suitable floatation solution, along with optimal centrifugation speed and time for Eimeria tenella (E. tenella) oocyst and sporocyst purification. Different floatation solutions i-e, saturated salt, Sheather's sugar and sodium hypochlorite (NaClO) at 20-60% concentrations were used to purify oocyst. It was found that about 96.99% oocysts (8609xg for 10 min) were recovered under these conditions without any effect on the viability of sporocysts. The recovery rate of oocysts using 50% NaClO (V/V) was significantly higher than 35% saturated salt flotation solution (P < 0.05). The optimal method for purification of oocysts based our experimentation was centrifugation at 8609xg for 3 min using 50% NaClO floatation solution, and the optimized centrifugation conditions for improved recovery of sporocysts (about 99.3%) were at 2152xg for 5 min. The present study provided a better method for the coccidian oocyst purification, which could be successfully adopted as a better alternative to existing techniques commonly used for investigations/research pertaining to coccidia.
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