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Inhibitive Mechanism of Loquat Flower Isolate on Tyrosinase Activity and Melanin Synthesis in Mouse Melanoma B16 Cells

文献类型： 外文期刊

作者： Chen, Qianqian 1 ; Tao, Wenyang 1 ; Wang, Jianfeng 3 ; Li, Jingrui 1 ; Zheng, Meiyu 1 ; Liu, Yinying 4 ; Lu, Shengmin 1 ; Fang, Zhongxiang 4 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Food Sci, State Key Lab Managing Biot & Chem Threats Qual &, Zhejiang Prov Key Lab Fruit & Vegetables Postharve, Hangzhou 310021, Peoples R China

2.Zhejiang Normal Univ, Coll Life Sci, Jinhua 321004, Peoples R China

3.Zhejiang Normal Univ, Xingzhi Coll, Lanxi 321100, Peoples R China

4.Univ Melbourne, Sch Agr Food & Ecosyst Sci, Parkville, Vic 3010, Australia

关键词： loquat flower isolate; tyrosinase inhibitory activity; melanin synthesis; melanoma B16 cells; multi-spectroscopy; Western blotting

期刊名称：BIOMOLECULES （ 影响因子：4.8； 五年影响因子：5.6 ）

ISSN：

年卷期： 2024 年 14 卷 8 期

页码：

收录情况： SCI

摘要： Melanin naturally exists in organisms and is synthetized by tyrosinase (TYR); however, its over-production may lead to aberrant pigmentation and skin conditions. Loquat (Eriobotrya japonica (Thunb.) Lindl.) flowers contain a variety of bioactive compounds, while studies on their suppressive capabilities against melanin synthesis are limited. Loquat flower isolate product (LFP) was obtained by ethanol extraction and resin purification, and its inhibitory efficiency against TYR activity was investigated by enzyme kinetics and multiple spectroscopy analyses. In addition, the impact of LFP on melanin synthesis-related proteins' expression in mouse melanoma B16 cells was analyzed using Western blotting. HPLC-MS/MS analysis indicated that LFP was composed of 137 compounds, of which 12 compounds, including flavonoids (quercetin, isorhamnoin, p-coumaric acid, etc.) and cinnamic acid and its derivatives, as well as benzene and its derivatives, might have TYR inhibitory activities. LFP inhibited TYR activity in a concentration-dependent manner with its IC50 value being 2.8 mg/mL. The inhibition was an anti-competitive one through altering the enzyme's conformation rather than chelating copper ions at the active center. LFP reduced the expression of TYR, tyrosinase-related protein (TRP) 1, and TRP2 in melanoma B16 cells, hence inhibiting the synthesis of melanin. The research suggested that LFP had the potential to reduce the risks of hyperpigmentation caused by tyrosinase and provided a foundation for the utilization of loquat flower as a natural resource in the development of beauty and aging-related functional products.

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