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Identification of loci and candidate gene GmSPX-RING1 responsible for phosphorus efficiency in soybean via genome-wide association analysis

文献类型： 外文期刊

作者： Du, Wenkai 1 ; Ning, Lihua 2 ; Liu, Yongshun 1 ; Zhang, Shixi 1 ; Yang, Yuming 1 ; Wang, Qing 1 ; Chao, Shengqian 1 ; Yan 1 ;

作者机构： 1.Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Natl Ctr Soybean Improvement, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Inst Crop Germplasm & Biotechnol, Nanjing 210014, Peoples R China

3.Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Peoples R China

关键词： Soybean; GWAS; P efficiency; GmSPX-RING1; P concentration

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundPhosphorus (P) is an essential element in maintaining high biomass and yield in crops. Soybean [Glycine max (L.) Merr.] requires a large amount of P during growth and development. Improvement of P efficiency and identification of P efficiency genes are important strategies for increasing soybean yield.ResultsGenome-wide association analysis (GWAS) with NJAU 355K SoySNP array was performed to identify single nucleotide polymorphisms (SNPs) significantly associated with three shoot P efficiency-related traits of a natural population of 211 cultivated soybeans and relative values of these traits under normal P (+P) condition and P deficiency (-P) condition. A total of 155 SNPs were identified significantly associated with P efficiency-related traits. SNPs that were significantly associated with shoot dry weight formed a SNP cluster on chromosome 11, while SNPs that were significantly associated with shoot P concentration formed a SNP cluster on chromosome 10. Thirteen haplotypes were identified based on 12 SNPs, and Hap9 was considered as the optimal haplotype. Four SNPs (AX-93636685, AX-93636692, AX-93932863, and AX-93932874) located on chromosome 10 were identified to be significantly associated with shoot P concentration under +P condition in two hydroponic experiments. Among these four SNPs, two of them (AX-93636685 and AX-93932874) were also significantly associated with the relative values of shoot P concentration under two P conditions. One SNP AX-93932874 was detected within 5-untranslated region of Glyma.10g018800, which contained SPX and RING domains and was named as GmSPX-RING1. Furthermore, the function research of GmSPX-RING1 was carried out in soybean hairy root transformation. Compared with their respective controls, P concentration in GmSPX-RING1 overexpressing transgenic hairy roots was significantly reduced by 32.75% under +P condition; In contrast, P concentration in RNA interference of GmSPX-RING1 transgenic hairy roots was increased by 38.90 and 14.51% under +P and -P conditions, respectively.Conclusions This study shows that the candidate gene GmSPX-RING1 affects soybean phosphorus efficiency by negatively regulating soybean phosphorus concentration in soybean hairy roots. The SNPs and candidate genes identified should be potential for improvement of P efficiency in future soybean breeding programs.

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