Molecular cloning and functional characterization of a thioredoxin peroxidase gene in Echinococcus multilocularis
文献类型： 外文期刊
作者： Guo, Xiaola 1 ; Zhang, Junmei 1 ; Li, Rui 1 ; Li, Hong 1 ; He, Xuedong 1 ; Wang, Shuai 1 ; Wang, Zhengrong 2 ; Harandi, M 1 ;
作者机构： 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China
2.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Peoples R China
3.Kerman Univ Med Sci, Res Ctr Hydatid Dis Iran, Dept Parasitol, Kerman, Iran
4.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China
5.Yangzhou Univ, Coll Vet Med, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
关键词： Echinococcus multilocularis; Thioredoxin peroxidase; Peroxidase activity; Oxidative stress; M2 macrophage
期刊名称：MOLECULAR AND BIOCHEMICAL PARASITOLOGY （ 影响因子：1.759； 五年影响因子：2.135 ）
ISSN： 0166-6851
年卷期： 2021 年 245 卷
页码：
收录情况： SCI
摘要： Thioredoxin peroxidase (TPx) plays an important role in protecting parasites against oxidative damage. However, studies on the role of TPxs in Echinococcus multilocularis are limited. In this study, one tpx gene of E. multilocularis, named as emtpx-1, was identified. EmTPx-1 shares two positionally conserved cysteine residues (Cys48 and Cys169) with orthologs from other platyhelminths. EmTPx-1 is highly expressed in the germinal layer and present in exosome-like vesicles secreted by E. multilocularis metacestodes. EmTPx-1 displays peroxidase activity, which removes hydrogen peroxide in the presence of dithiothreitol. Furthermore, EmTPx-1 could protect DNA from oxidative damages, and EmTPx-1-expressing E. coli cells had an enhanced resistance to oxidative stress. In addition, EmTPx-1 enhanced the expression of arg1, ym1, and il-10, but suppressed inos, tnf-alpha, and il-1 beta expression in LPS-stimulated macrophages. Our data suggest a critical role for EmTPx-1 in oxidative stresses and M2 macrophage polarization.
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