文献类型： 外文期刊
作者： Tan, Jiantao 1 ; Shen, Mengyuan 1 ; Chai, Nan 2 ; Liu, Qi 1 ; Liu, Yao-Guang 2 ; Zhu, Qinlong 2 ;
作者机构： 1.Guangdong Acad Agr Sci, Rice Res Inst, Guangdong Key Lab New Technol Rice Breeding, Minist Agr & Rural Affairs,Guangdong Rice Engn Lab, Guangzhou 510640, Peoples R China
2.South China Agr Univ, Coll Life Sci, Guangdong Lab Lingnan Modern Agr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Peoples R China
关键词： Genome editing; Synthetic metabolic engineering; Developmental regulation; Plant breeding
期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：4.3； 五年影响因子：4.5 ）
ISSN： 0176-1617
年卷期： 2023 年 291 卷
页码：
收录情况： SCI
摘要： Plant metabolism and development are a reflection of the orderly expression of genetic information intertwined with the environment interactions. Genome editing is the cornerstone for scientists to modify endogenous genes or introduce exogenous functional genes and metabolic pathways, holding immense potential applications in molecular breeding and biosynthesis. Over the course of nearly a decade of development, genome editing has advanced significantly beyond the simple cutting of double-stranded DNA, now enabling precise base and fragment replacements, regulation of gene expression and translation, as well as epigenetic modifications. However, the utilization of genome editing in plant synthetic metabolic engineering and developmental regulation remains exploratory. Here, we provide an introduction and a comprehensive overview of the editing attributes associated with various CRISPR/Cas tools, along with diverse strategies for the meticulous control of plant metabolic pathways and developments. Furthermore, we discuss the limitations of current approaches and future prospects for genome editing-driven plant breeding.
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