文献类型： 外文期刊
作者： Kern, Chandlar 1 ; Wu, Weiwei 1 ; Lu, Chen 1 ; Zhang, Jianbin 1 ; Zhao, Yaqi 1 ; Ocon-Grove, Olga Maria 1 ; Sutovsky, Peter 4 ; Diaz, Francisco 1 ; Liu, Wan-Sheng 1 ;
作者机构： 1.Penn State Univ, Coll Agr Sci, Ctr Reprod Biol & Hlth CRBH, Dept Anim Sci, 311 AVBS Bldg, University Pk, PA 16802 USA
2.Xinjiang Acad Agr Sci, Anim Sci Inst, Xinjiang, Peoples R China
3.Tianjin Agr Univ, Dept Anim Sci, Tianjin, Peoples R China
4.Univ Missouri, Div Anim Sci, Gynecol & Womens Hlth, Columbia, MO USA
5.Univ Missouri, Dept Obstet, Gynecol & Womens Hlth, Columbia, MO USA
6.Fudan Univ, Shanghai, Peoples R China
7.St Jude Childrens Res Hosp, Memphis, TN USA
8.Actuated Med Inc, Bellefonte, PA USA
关键词： PRAMEY; Sperm capacitation; Acrosomal exocytosis; In vitro fertilization; Bovine
期刊名称：CELL AND TISSUE RESEARCH （ 影响因子：4.051； 五年影响因子：5.027 ）
ISSN： 0302-766X
年卷期：
页码：
收录情况： SCI
摘要： Preferentially expressed antigen in melanoma (PRAME) is a cancer/testis antigen (CTA) that is predominantly expressed in normal male gonad tissues and a variety of tumors. PRAME proteins are present in the acrosome and sperm tail, but their role in sperm function is unknown. The objective of this study was to examine the function of the bovine Y-linked PRAME (PRAMEY) during spermatozoal capacitation, the acrosome reaction (AR), and fertilization. Freshly ejaculated spermatozoa were induced to capacitate and undergo AR in vitro. Western blotting results revealed a decrease in the PRAMEY protein in capacitated spermatozoa, and the release of the PRAMEY protein from the acrosome during the AR, suggesting its involvement in sperm capacitation and AR. IVF was performed using in vitro matured bovine oocytes and cauda epididymal spermatozoa either treated with PRAMEY antibody, rabbit IgG, or DPBS. Sperm-egg binding and early embryos were examined at 6 and 45 h post IVF, respectively. The number of spermatozoa that bound per oocyte was nearly two-fold greater in the PRAMEY antibody treatment group (34.4) when compared to both the rabbit IgG (17.6) and DPBS (18.1) controls (P < 0.01). Polyspermy rate in the antibody-treated group (18.9%) was three-fold greater than the rabbit IgG control (6.0%) (P < 0.01). The results indicate that PRAMEY may play a role in anti-polyspermy defense. This study thus provides the initial evidence for the involvement of the PRAME protein family in sperm function and fertilization.
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