Coupled magnetic nanoparticle-mediated isolation and single-cell image recognition to detect Bacillus' cell size in soil
文献类型： 外文期刊
作者： Rong, Nan 1 ; He, Shiying 3 ; Li, Bei 5 ; Lin, Xiangui 1 ; Liu, Xiaoling 7 ; Yu, Yongjie 1 ; Feng, Youzhi 1 ;
作者机构： 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China
2.Univ Chinese Acad Sci, Sch Resources & Environm, Beijing, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjing, Peoples R China
4.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang, Jiangsu, Peoples R China
5.Chinese Acad Sci, Changchun Inst Opt Fine Mech & Phys, Changchun, Peoples R China
6.HOOKE Instruments Ltd, Changchun, Peoples R China
7.Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing, Peoples R China
8.Chinese Acad Geol Sci, Inst Karst Geol, MNR & Guangxi Key Lab Karst Dynam, Guilin, Peoples R China
关键词： Bacillus; cell size; fertilisation; magnetic nanoparticle-mediated isolation; single-cell image recognition
期刊名称：EUROPEAN JOURNAL OF SOIL SCIENCE （ 影响因子：4.178； 五年影响因子：4.883 ）
ISSN： 1351-0754
年卷期： 2022 年 73 卷 3 期
页码：
收录情况： SCI
摘要： Microbial morphology fundamentally constrains how species interact with their environment, and hence ultimately affects their niche. However, the methodology of functional microbes in the soil ecosystem is still poorly studied since it is difficult to capture and identify the active monospecific community from the complicated environment and enormous number of microbial species in soils. To comprehensively reveal the morphology of active microbes in soil ecosystem, magnetic nanoparticle-mediated isolation (MMI) and single-cell image recognition (SCIR) were employed to study soil active Bacillus community, which functionally boosted the soil fertility in organic fertilisation compared to mineral fertilisation and unfertilised control treatments in our previous study. The results showed that MMI and SCIR can efficiently isolate active Bacillus from soil particles and other microorganisms. High throughput sequencing showed that the captured Bacillus showed similar community structure in different long-term fertilisation soils, while SCIR revealed that the active Bacillus was greater in number and larger in size in organic fertilisation treatment compared to mineral fertilisation and unfertilised control treatments. Our study demonstrates that the combination of MMI and SCIR is a potentially powerful tool to capture and identify the morphology of active and functional microbes in the soil ecosystem.
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