Propagation of pineapple (Ananas comosus L.) embryogenic cell suspension is regulated by LEAFY COTYLEDON1 gene AcoLEC1-1
文献类型： 外文期刊
作者： Shu, Haiyan 1 ; Zhan, Rulin 1 ; Eissa, Mamdouh A. 4 ; Vafadar, Farinaz 6 ; Ding, Zheli 2 ; Wang, You 1 ; He, Junhu 1 ; Wei, Qing 1 ; Luan, Aiping 1 ; Chang, Shenghe 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572025, Hainan, Peoples R China
3.Natl Key Lab Trop Crop Breeding, Sanya 572025, Hainan, Peoples R China
4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 572025, Hainan, Peoples R China
5.Assiut Univ, Fac Agr, Dept Soils & Water, Assiut 71526, Egypt
6.Isfahan Univ Technol, Coll Agr, Dept Agron & Plant Breeding, Esfahan 8415683111, Iran
关键词： Pineapple; Optimum medium; Sucrose; pH; ECS; LEC1; SUT
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.3； 五年影响因子：4.5 ）
ISSN： 0304-4238
年卷期： 2024 年 332 卷
页码：
收录情况： SCI
摘要： Pineapple (Ananas comosus L.) is an important tropical fruit. Traditional breeding methods take about 15 years, but transgenic breeding using embryogenic cell suspension (ECS) can significantly shorten this cycle. Four different calli types were produced using the pineapple crown's middle stem part as an explant: water-like, solid, gemmiform, and crisp. The fastest multiplication was observed in suspension cultures obtained from the loose, crispy, granular, white, or yellow callus. The highest cell density in the liquid medium was achieved with MS+1.5 mg/L+4 mg/L6-BA+300 mg/L hormone combination. Inclusion of 11 mmol/L 2-morpholinoethanesulfonic acid (MES) stabilized the medium pH. The optimum sucrose concentration for pineapple propagation using ECS was 5 g/L. The expression of seven proteins in ECS in the medium containing 5 g/L sucrose was more than 5.0 times higher than in the medium containing 3 g/L. The expression of transcription factor NFYB/HAP3 (or LEAFY COTYLEDON1, LEC1) was the highest in the medium. The most prevalent AcoLEC1-1 transcripts were found in the embryogenic callus, and their patterns correlated well with ECS volume. AcoLEC1-1 played a key role in the process of sucrose-regulated ECS propagation.
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