文献类型： 外文期刊
作者： Lin, Fei 1 ; Kang, Yong 1 ; Li, Yamei 1 ; Guo, Yuhua 1 ; Wang, Wei 3 ; Yang, Guangsui 1 ; Yin, Junmei 1 ; Tang, Fenling 1 ; Eissa, Mamdouh A. 2 ;
作者机构： 1.Minist Agr & Rual Affairs, Engn Technol Res Ctr Trop Ornamental Plant Germpla, Key Lab Trop Crops Germplasm Resources Genet Impro, Trop Crops Genet Resources Inst,CATAS,Key Lab Crop, Haikou 571101, Peoples R China
2.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou 571101, Peoples R China
3.Hainan Univ, Sch Agr, Haikou 571101, Peoples R China
4.CATAS, Sanya Res Inst, Natl Key Lab Trop Crop Breeding, Sanya 572024, Peoples R China
5.Assiut Univ, Fac Agr, Dept Soils & Water, Assiut 71526, Egypt
关键词： tissue culture; Nymphoides; plant growth regulators; 6-BA; IAA; NAA
期刊名称：PLANTS-BASEL （ 影响因子：4.0； 五年影响因子：4.4 ）
ISSN： 2223-7747
年卷期： 2024 年 13 卷 11 期
页码：
收录情况： SCI
摘要： Nymphoides coronata is an endangered aquatic plant species with significant medicinal and ecological importance. To preserve N. coronata from going extinct, we need to provide seedlings and efficient multiplication techniques so that it can be extensively studied. This study aimed to identify the most suitable sterilization treatment, growth medium, and substrate for the cultivation and propagation of N. coronata. Ethanol sterilization, fungicide treatment, and sterile water washing were the most important sterilization steps. A combination of 6-benzylaminopurine (6-BA) and indoleacetic acid (IAA) was the most suitable medium for bud induction and shoot proliferation. The use of alpha-naphthaleneacetic acid (NAA) increased the rooting rate and rooting time compared to indole-3-butyric acid (IBA). Increasing the concentration of NAA from 0.5 to 1.0 mg/L increased the rooting rate from 78 to 100% and reduced the rooting time from 7 to 5 days. The survival rate of N. coronata seedlings was 100% in a mixture of red soil and sand (1:1, w/w). As a result, the procedure mentioned above could potentially be used to safely propagate this rare species on a large scale. These findings provide valuable insights into the optimal conditions for the successful cultivation and propagation of N. coronata, which can contribute to the conservation and sustainable use of this important rare plant species.
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