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iTRAQ-Based Quantitative Proteomics Reveals ChAcb1 as a Novel Virulence Factor in Colletotrichum higginsianum

文献类型： 外文期刊

作者： Yan, Yaqin 1 ; Tang, Jintian 3 ; Yuan, Qinfeng 1 ; Liu, Liping 4 ; Liu, Hao 1 ; Huang, Junbin 1 ; Hsiang, Tom 5 ; Zheng, Lu 1 ;

作者机构： 1.Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Vegetables Res, Hangzhou 310021, Peoples R China

3.China Jiliang Univ, Coll Life Sci, Zhejiang Prov Key Lab Biometrol & Inspect & Quara, Hangzhou 310018, Peoples R China

4.Jilin Agr Univ, Dept Agron, Lab Plant Pathol, Changchun 130118, Peoples R China

5.Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

关键词： ChAcb1; Colletotrichum higginsianum; iTRAQ; virulence

期刊名称：PHYTOPATHOLOGY （ 影响因子：4.01； 五年影响因子：4.457 ）

ISSN： 0031-949X

年卷期： 2021 年 111 卷 9 期

页码：

收录情况： SCI

摘要： Colletotrichum higginsianum is an important hemibiotrophic fungal pathogen that causes anthracnose disease on various cruciferous plants. Discovery of new virulence factors could lead to strategies for effectively controlling anthracnose. Acyl-CoA binding proteins (ACBPs) are mainly involved in binding and trafficking acyl-CoA esters in eukaryotic cells. However, the functions of this important class of proteins in plant fungal pathogens remain unclear. In this study, we performed an isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomic analysis to identify differentially expressed proteins (DEPs) between a nonpathogenic mutant ACh-MEL1 and the wild type. Based on iTRAQ data, DEPs in the ACh-MEL1 mutant were mainly associated with melanin biosynthesis, carbohydrate and energy metabolism, lipid metabolism, redox processes, and amino acid metabolism. Proteomic analysis revealed that many DEPs might be involved in growth and pathogenesis of C. higginsianum. Among them, an acyl-CoA binding protein, ChAcb1, was selected for further functional studies. Deletion of ChAcb1 caused defects in vegetative growth and conidiation. ChAcb1 is also required for response to hyperosmotic and oxidative stresses, and maintenance of cell wall integrity. Importantly, the AChAcb1 mutant exhibited reduced virulence, and microscopic examination revealed that it was defective in appressorial penetration and infectious growth. Furthermore, the AChAcb1 mutant was impaired in fatty acid and lipid metabolism. Taken together, ChAcb1 was identified as a new virulence gene in this plant pathogenic fungus.

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