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Bacillus subtilis phage phi18: genomic analysis and receptor identification

文献类型： 外文期刊

作者： Zhang, Zhiqiang 1 ; Liang, Li 2 ; Li, Donghang 1 ; Li, Yutong 1 ; Sun, Qinghui 3 ; Li, Ye 4 ; Yang, Hongjiang 1 ;

作者机构： 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Key Lab Ind Microbiol, Tianjin Key Lab Ind Microbiol,Minist Educ, Tianjin 300457, Peoples R China

2.Shandong Vland Biotech Co Ltd, Qingdao 251700, Shandong, Peoples R China

3.Hainan Med Univ, Sch Trop Med, Key Lab Trop Translat Med, NHC Key Lab Control Trop Dis,Minist Educ, Hainan 571199, Peoples R China

4.Hainan Univ, Inst Environm & Plant Protect, Chinese Acad Trop Agr Sci, Hainan 571199, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.7； 五年影响因子：2.4 ）

ISSN： 0304-8608

年卷期： 2023 年 168 卷 1 期

页码：

收录情况： SCI

摘要： Bacillus subtilis strains play a pivotal role in the fermentation industry. B. subtilis phages can cause severe damage by infecting bacterial cells used in industrial fermentation processes. In this work, we isolated and characterized a Bacillus subtilis-infecting phage, termed phi18. Transmission electron microscopy revealed that phage phi18 particles have typical myovirus morphology, with an icosahedral head connected to a contractile tail. Genomic analysis revealed that the phage genome is a linear double-stranded DNA molecule of 147,298 bp with terminal redundancy of 14,434 bp, and 226 protein coding genes and four tRNA genes were predicted in the genome. Phage-resistant mutants were selected from a mariner transposon-insertion library of B. subtilis 168 in which two bacterial genes, tagE and pgcA, which are required for the glycosylation of wall teichoic acid (WTA), were found to be disrupted, suggesting that WTA is the receptor for phage phi18. Comparative genomic analysis showed that phage phi18 is a new member of the genus Okubovirus of the family Herelleviridae. Finally, general characteristics of the phage-resistant mutants, including biofilm formation, growth, and sporulation, were examined. The results showed that the phage-resistant mutants grew as rapidly as the parental strain B. subtilis 168 at 42 & DEG;C, suggesting that these phage-resistant mutants may be used as starters in fermentation processes.

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